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锟斤拷隆锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟斤拷(w锟斤拷)锟斤拷(ji锟斤拷n)锟斤拷
锟斤拷隆锟斤拷(g锟斤拷u)锟斤拷锟角凤拷锟斤拷锟斤拷锟斤拷W(xu锟斤拷)锟叫撅拷锟斤拷锟筋常锟矫碉拷锟斤拷锟街讹拷之一,锟斤拷锟斤拷锟斤拷锟斤拷影锟斤拷锟斤拷锟斤拷^锟洁，锟斤拷(du锟斤拷)锟叫撅拷锟竭斤拷(j锟斤拷ng)锟(y锟斤拷n)要锟斤拷锟絕锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷M(f锟斤拷i)锟絩(sh锟斤拷)锟組(f锟斤拷i)锟斤拷,锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟絳锟斤拷锟絃(zh锟斤拷ng)锟节忥拷锟铰凤拷锟斤拷锟斤拷锟斤拷W(xu锟斤拷)锟斤拷锟斤拷锟斤拷锟絊锟斤拷锟斤拷(j锟斤拷ng)锟(y锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟结供锟斤拷锟斤拷效,锟斤拷锟斤拷锟斤拷莸姆锟斤拷锟(w锟斤拷),锟斤拷锟斤拷锟斤拷锟斤拷隆锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絇(gu锟斤拷n)锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷Q锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷(j锟斤拷)目锟侥伙拷锟斤拷膩锟(l锟斤拷i)源,锟斤拷锟絗锟斤拷锟絰锟斤拷同锟侥匡拷隆锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟侥伙拷锟斤拷M锟叫凤拷锟絰目锟侥伙拷锟斤拷锟斤拷锟截讹拷mRNA锟斤拷锟紻(zhu锟斤拷n)錄合筹拷cDNA锟斤拷锟斤拷锟組(j锟斤拷n)锟叫匡拷隆锟斤拷PCR锟絯锟斤拷U(ku锟斤拷)锟斤拷目锟斤拷片锟斤拷锟組(j锟斤拷n)锟叫匡拷隆锟斤拷,锟斤拷
锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟结供锟斤拷T锟絛锟絯锟斤拷锟斤拷锟(l锟斤拷i)锟斤拷pUC18/19锟斤拷锟斤拷pJET1.2, 锟紹锟接時(sh锟斤拷)锟絞锟斤拷,锟斤拷锟紻(zhu锟斤拷n)锟斤拷效锟绞高★拷一锟斤拷2锟斤拷锟斤拷锟揭匡拷锟结供锟斤拷锟斤拷锟統(c锟斤拷)锟斤拷蕡D,锟斤拷锟截斤拷锟絴(zh锟斤拷)锟斤拷锟饺岋拷(sh锟斤拷)锟(y锟斤拷n)锟結(ji锟斤拷)锟斤拷锟侥岋拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷,锟斤拷
锟斤拷锟斤拷锟絛锟絯锟斤拷pJET1.2/bulnt锟饺ｏ拷锟紻锟絍锟斤拷锟铰ｏ拷
锟斤拷隆锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1,锟斤拷锟絔(m锟斤拷i)锟斤拷模锟斤拷r(sh锟斤拷),锟斤拷請(q锟斤拷ng)?zh锟斤拷)峁╋拷锟斤拷锟饺NA锟斤拷锟斤拷RNA锟侥憋拷锟斤拷锟斤拷玫臉颖锟斤拷锟斤拷希锟
2,锟斤拷锟斤拷锟斤拷锟侥ｏ拷锟,锟斤拷請(q锟斤拷ng)?zh锟斤拷)峁╋拷锟斤拷锟斤拷?锟斤拷g锟斤拷PCR锟絘(ch锟斤拷n)锟斤，锟斤拷锟斤拷锟組DNA锟斤拷1锟斤拷g,锟斤拷锟斤拷锟絴(zh锟斤拷)锟,锟斤拷锟100ng锟斤拷锟斤拷cDNA锟斤拷品锟斤拷10锟斤拷l
3,锟斤拷锟结供锟斤拷锟斤拷隆锟斤拷锟斤拷锟狡拷蔚锟斤拷锟斤拷谢锟斤拷锟教(h锟斤拷o),锟斤拷
锟斤拷隆锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1. 锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟組(j锟斤拷n)锟斤拷锟斤拷锟斤拷锟絆(sh锟斤拷)計(j锟斤拷)锟较筹拷锟斤拷锟斤；
2. PCR锟斤拷锟紹锟斤拷,锟斤拷
3. 锟統(c锟斤拷)锟斤拷锟絙锟斤拷锟斤拷酶锟斤拷锟絙锟斤拷,锟斤拷
锟斤拷隆锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟斤拷锟斤拷(n锟斤拷i)锟斤拷
锟絴(zh锟斤拷)锟斤拷锟斤拷锟揭,锟斤拷锟統(c锟斤拷)锟斤拷锟(b锟斤拷o)锟斤拷,锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷
锟斤拷隆锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟斤拷(w锟斤拷)锟斤拷(n锟斤拷i)锟斤拷
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