| 锟斤拷品锟斤拷锟 |
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟 |
锟酵橈拷锟斤拷 |
锟絓輸锟斤拷(bi锟斤拷o)锟斤拷(zh锟斤拷n) |
| 锟斤拷植锟斤拷M锟斤拷,锟斤拷锟斤拷锟絯 |
锟斤拷锟斤拷MDNA锟斤拷取锟斤拷RNA锟斤拷取,锟斤拷miRNA锟斤拷取 |
锟斤拷100mg |
锟缴憋拷锟絓輸 |
| 锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷 |
锟斤拷锟斤拷MDNA锟斤拷取,锟斤拷RNA锟斤拷取锟斤拷miRNA锟斤拷取 |
锟斤拷10锟斤拷7锟轿凤拷cells |
锟缴憋拷锟絓輸 |
| 血锟接ｏ拷锟斤拷锟斤拷锟斤拷铮 |
锟斤拷锟斤拷MDNA锟斤拷取 |
500ul |
锟缴憋拷锟絓輸 |
| RNA锟斤拷取 |
100-200ul血液锟秸硷拷锟侥白硷拷锟斤拷 |
锟斤拷锟斤拷1ml trizol锟缴憋拷锟絓輸 |
| 锟斤拷液 |
锟絴(zh锟斤拷)锟斤拷DNA锟斤拷取,锟斤拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷锟斤拷 |
锟斤拷锟酵撅拷液锟斤拷500ul |
锟斤拷锟斤拷锟絓輸 |
| 锟絴(zh锟斤拷)锟斤拷DNA |
锟絴(zh锟斤拷)锟斤拷DNA锟斤拷取 |
锟斤拷100ng |
锟斤拷锟斤拷锟斤拷杀锟斤拷\輸 |
| PCR,锟斤拷qPCR锟斤拷锟斤拷锟斤拷锟絚突變,锟斤拷原锟剿碉拷锟斤拷小锟斤拷锟斤拷锟絖,锟斤拷锟斤拷母锟斤拷锟斤拷小锟斤拷锟斤拷锟絖锟斤拷锟斤拷锟斤拷锟斤拷锓(w锟斤拷n)锟斤拷锟紻(zhu锟斤拷n)染锟斤拷锟斤拷系锟斤拷(g锟斤拷u)锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絙,锟斤拷锟絧锟缴癸拷锟斤拷酶锟斤拷锟斤拷锟斤拷z锟統 |
锟斤拷2ug |
| 锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷 |
锟斤拷10ug |
| 锟斤拷锟斤拷MDNA |
PCR锟斤拷qPCR |
锟斤拷2ug |
锟斤拷锟斤拷锟斤拷杀锟斤拷\輸 |
| Total RNA |
锟斤拷锟紻(zhu锟斤拷n)锟 |
锟斤拷5ug |
锟缴憋拷锟絓輸 |
| PCR锟斤拷锟斤拷锟絘(ch锟斤拷n)锟斤拷锟斤拷z锟斤拷锟秸產(ch锟斤拷n)锟斤拷 |
锟斤拷锟斤拷锟铰 |
锟絯锟絜锟斤拷50ul,锟斤拷取锟50ng/ul
锟斤拷锟斤拷锟斤拷2ug |
锟斤拷锟斤拷锟斤拷杀锟斤拷\輸 |
| 目锟斤拷片锟斤拷锟絚锟斤拷锟紹锟接碉拷锟絛锟絯 |
锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷 |
目锟斤拷片锟轿伙拷锟斤拷片锟轿碉拷锟絛锟絯锟斤拷5ug,锟斤拷锟紹锟接碉拷锟絛锟絯锟斤拷10ug |
锟斤拷锟斤拷锟斤拷杀锟斤拷\輸 |
| 锟斤拷锟阶橈拷品 |
锟斤拷锟斤拷PAGE锟絲锟統
Wstern BLot
|
锟斤拷锟斤拷锟斤拷100ug
锟斤拷取锟2ug/ul
|
锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷呀锟揭猴拷锟,锟斤拷請锟接碉拷锟斤拷酶锟斤拷锟狡勶拷,锟斤拷然锟斤拷杀锟斤拷\輸锟斤拷
锟斤拷锟斤拷锟斤拷锟斤拷呀锟(j锟斤拷ng)锟斤拷SDS-PAGE loading buffer锟斤拷,锟斤拷锟斤拷锟斤拷锟絓輸锟斤拷
|
| 锟斤拷锟斤拷系 |
锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng),锟斤拷锟斤拷锟斤拷锟斤拷锛氾拷锟斤拷锟斤拷妆锟斤拷_,锟斤拷锟斤拷锟斤拷锟斤拷锓(w锟斤拷n)锟斤拷锟紻(zhu锟斤拷n)染锟斤拷锟斤拷系锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟絧锟缴癸拷锟斤拷酶锟斤拷锟斤拷锟斤拷z锟統,锟斤拷锟斤拷锟斤拷锟斤拷殖锟酵硷拷锟斤拷锟斤拷锟皆檢锟統 |
锟斤拷3*10锟斤拷6锟轿凤拷cells |
锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷:锟斤拷锟斤拷
锟斤拷锟芥細锟斤拷:锟缴憋拷 |
