| 锟斤拷品锟斤拷锟 |
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟 |
锟酵橈拷锟斤拷 |
锟絓(y锟斤拷n)輸锟斤拷(bi锟斤拷o)锟斤拷(zh锟斤拷n) |
| 锟斤拷(d锟斤拷ng)植锟斤拷M锟斤拷,锟斤拷锟斤拷锟絯 |
锟斤拷锟斤拷MDNA锟斤拷取锟斤拷RNA锟斤拷取,锟斤拷miRNA锟斤拷取 |
锟斤拷100mg |
锟缴憋拷锟絓(y锟斤拷n)輸 |
| 锟斤拷锟紹(y锟斤拷ng)锟斤拷(x锟斤拷)锟斤拷 |
锟斤拷锟斤拷MDNA锟斤拷取,锟斤拷RNA锟斤拷取锟斤拷miRNA锟斤拷取 |
锟斤拷10锟斤拷7锟轿凤拷cells |
锟缴憋拷锟絓(y锟斤拷n)輸 |
| 血锟接ｏ拷锟斤拷锟斤拷锟(d锟斤拷ng)锟斤） |
锟斤拷锟斤拷MDNA锟斤拷取 |
500ul |
锟缴憋拷锟絓(y锟斤拷n)輸 |
| RNA锟斤拷取 |
100-200ul血液锟秸硷拷锟侥白硷拷(x锟斤拷)锟斤拷 |
锟斤拷锟斤拷1ml trizol锟缴憋拷锟絓(y锟斤拷n)輸 |
| 锟斤拷液 |
锟絴(zh锟斤拷)锟斤拷DNA锟斤拷取,锟斤拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷锟斤拷 |
锟斤拷锟酵撅拷液锟斤拷500ul |
锟斤拷锟斤拷锟絓(y锟斤拷n)輸 |
| 锟絴(zh锟斤拷)锟斤拷DNA |
锟絴(zh锟斤拷)锟斤拷DNA锟斤拷取 |
锟斤拷100ng |
锟斤拷锟斤拷锟斤拷杀锟斤拷\(y锟斤拷n)輸 |
| PCR,锟斤拷qPCR锟斤拷锟斤拷锟斤拷锟絚(di锟斤拷n)突變,锟斤拷原锟剿碉拷锟斤拷小锟斤拷锟斤拷锟絖(d锟斤拷),锟斤拷锟斤拷母锟斤拷锟斤拷小锟斤拷锟斤拷锟絖(d锟斤拷)锟斤拷锟斤拷锟斤拷锟(d锟斤拷ng)锟斤穩(w锟斤拷n)锟斤拷锟紻(zhu锟斤拷n)染锟斤拷(x锟斤拷)锟斤拷系锟斤拷(g锟斤拷u)锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絙,锟斤拷锟絧锟缴癸拷锟斤拷酶锟斤拷(b锟斤拷o)锟斤拷锟斤拷锟絲锟統(c锟斤拷) |
锟斤拷2ug |
| 锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷 |
锟斤拷10ug |
| 锟斤拷锟斤拷MDNA |
PCR锟斤拷qPCR |
锟斤拷2ug |
锟斤拷锟斤拷锟斤拷杀锟斤拷\(y锟斤拷n)輸 |
| Total RNA |
锟斤拷锟紻(zhu锟斤拷n)锟 |
锟斤拷5ug |
锟缴憋拷锟絓(y锟斤拷n)輸 |
| PCR锟斤拷锟斤拷锟絘(ch锟斤拷n)锟斤拷锟斤拷z锟斤拷锟秸產(ch锟斤拷n)锟斤拷 |
锟斤拷锟斤拷锟铰 |
锟絯锟絜锟斤拷50ul,锟斤拷取锟50ng/ul
锟斤拷锟斤拷锟斤拷2ug |
锟斤拷锟斤拷锟斤拷杀锟斤拷\(y锟斤拷n)輸 |
| 目锟斤拷片锟斤拷锟絚锟斤拷锟紹锟接碉拷锟絛锟絯 |
锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷 |
目锟斤拷片锟轿伙拷锟斤拷片锟轿碉拷锟絛锟絯锟斤拷5ug,锟斤拷锟紹锟接碉拷锟絛锟絯锟斤拷10ug |
锟斤拷锟斤拷锟斤拷杀锟斤拷\(y锟斤拷n)輸 |
| 锟斤拷锟阶橈拷品 |
锟斤拷锟斤拷PAGE锟絲锟統(c锟斤拷)
Wstern BLot
|
锟斤拷锟斤拷锟斤拷100ug
锟斤拷取锟2ug/ul
|
锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷呀锟揭猴拷锟,锟斤拷請(q锟斤拷ng)锟接碉拷锟斤拷酶锟斤拷锟狡勶拷,锟斤拷然锟斤拷杀锟斤拷\(y锟斤拷n)輸锟斤拷
锟斤拷锟斤拷锟斤拷锟斤拷呀锟(j锟斤拷ng)锟斤拷SDS-PAGE loading buffer锟斤拷,锟斤拷锟斤拷锟斤拷锟絓(y锟斤拷n)輸,锟斤拷
|
| 锟斤拷(x锟斤拷)锟斤拷系 |
锟斤拷(x锟斤拷)锟斤拷锟斤拷锟紹(y锟斤拷ng),锟斤拷锟斤拷锟斤拷锟(d锟斤拷ng)锟斤細(x锟斤拷)锟斤拷锟斤拷锟阶憋拷锟絖(d锟斤拷),锟斤拷锟斤拷锟斤拷锟(d锟斤拷ng)锟斤穩(w锟斤拷n)锟斤拷锟紻(zhu锟斤拷n)染锟斤拷(x锟斤拷)锟斤拷系锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟絧锟缴癸拷锟斤拷酶锟斤拷(b锟斤拷o)锟斤拷锟斤拷锟絲锟統(c锟斤拷),锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷殖锟酵硷拷(x锟斤拷)锟斤拷锟斤拷锟皆檢锟統(c锟斤拷) |
锟斤拷3*10锟斤拷6锟轿凤拷cells |
锟斤拷锟紹(y锟斤拷ng)锟斤拷(x锟斤拷)锟斤拷:锟斤拷锟斤拷
锟斤拷锟芥細(x锟斤拷)锟斤拷:锟缴憋拷 |
