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microRNA锟叫撅拷锟斤拷锟斤拷
MicroRNA (miRNA) 锟斤拷一锟(l锟斤拷i)锟缴冿拷(n锟斤拷i)源锟斤拷锟津編碼锟斤拷锟絃(zh锟斤拷ng)锟饺約锟斤拷22 锟斤拷(g锟斤拷)锟斤拷锟斤拷锟斤拷姆蔷锟斤拷a锟斤拷锟絉NA锟斤拷锟接ｏ拷锟斤拷锟斤拷?c锟斤拷)趧?d锟斤拷ng)植锟斤拷锟叫咃拷锟絚锟紻(zhu锟斤拷n)錄猴拷锟斤拷锟斤拷锟絖(d锟斤拷)锟絳(di锟斤拷o)锟斤拷,锟斤拷microRNA锟侥憋拷锟絖(d锟斤拷),锟斤拷锟斤拷锟狡伙拷锟絕(gu锟斤拷)锟斤拷锟絖(d锟斤拷)锟斤拷(du锟斤拷)锟斤拷锟叫撅拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷要锟斤拷锟斤拷锟絰锟斤拷目前锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷(du锟斤拷)锟斤拷microRNA锟叫撅拷锟斤拷锟絇(gu锟斤拷n)锟侥凤拷锟斤拷(w锟斤拷)锟斤拷锟铰ｏ拷
1. microRNA qPCR锟斤拷锟斤拷锟絲锟統(c锟斤拷)
锟絲锟統(c锟斤拷)microRNA锟侥差異锟斤拷锟絖(d锟斤拷),锟斤拷锟斤拷锟絅锟給(w锟斤拷)锟斤拷染锟斤拷锟铰诧拷锟皆橈拷品锟斤拷锟斤拷锟结供锟斤拷锟斤拷锟斤拷锟斤拷@锟斤拷锟狡,锟斤拷請(q锟斤拷ng)?zh锟斤拷)峁╋拷锟絉NA锟斤拷小RNA锟斤拷锟斤拷锟结供锟斤拷锟教(h锟斤拷o)(http://www.mirbase.org/ )锟斤拷microRNA锟斤拷锟斤拷,锟斤拷锟揭傦拷锟斤拷锟斤拷詳锟斤拷(x锟斤拷)锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷,锟斤拷锟給锟絟(hu锟斤拷n)锟斤拷锟斤拷锟轿诧拷锟斤拷锟斤拷D(zhu锟斤拷n)錄撅拷锟斤拷锟絰锟今，凤拷锟斤拷(w锟斤拷)锟斤拷锟紹锟斤拷锟斤拷锟铰ｏ拷
miRNA锟缴光定锟斤拷PCR
2. microRNA锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷
3. microRNA锟斤拷位锟絚(di锟斤拷n)锟結(ji锟斤拷)锟斤拷锟紸(y锟斤拷)锟統(c锟斤拷)锟斤拷锟絧锟缴癸拷锟斤拷酶锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷,锟斤拷锟缴癸拷锟斤拷酶锟絲锟統(c锟斤拷)
miRNA通锟絕(gu锟斤拷)锟紸锟斤拷锟斤拷锟絘(b锟斤拷)锟戒對(du锟斤拷)锟侥凤拷式锟絉(sh锟斤拷)锟絜锟叫伙拷锟斤拷锟3锟斤拷UTR锟絕(q锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷(j锟斤拷)锟斤拷锟絘(b锟斤拷)锟教度的诧拷同指锟斤拷(d锟斤拷o)锟斤拷默锟斤拷(f锟斤拷)锟斤拷锟絯锟斤拷锟斤拷锟絤RNA锟斤拷锟斤拷锟斤拷锟斤拷锟絤RNA锟侥凤拷锟絞,锟斤拷锟斤拷锟斤拷(j锟斤拷)軟锟斤拷锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷(k锟斤拷)锟紸(y锟斤拷)锟統(c锟斤拷)锟斤拷位锟絚(di锟斤拷n)锟斤拷锟斤拷(g锟斤拷u)锟斤拷锟斤拷位锟絚(di锟斤拷n)锟侥熒癸拷锟斤拷酶锟絛锟絯锟斤拷锟絲锟統(c锟斤拷),锟斤拷詳锟斤拷(x锟斤拷)锟斤拷B锟斤拷請(q锟斤拷ng)锟絚(di锟斤拷n)锟斤拷锟斤拷锟斤拷鏈接ｏ拷
锟絧锟缴癸拷锟斤拷酶锟斤拷(b锟斤拷o)锟斤拷锟斤拷锟絲锟統(c锟斤拷)锟斤拷miRNA锟斤拷(du锟斤拷)锟叫伙拷锟斤拷锟絳(di锟斤拷o)锟斤拷锟叫撅拷锟斤拷
4. microRNA锟紻(zhu锟斤拷n)染锟斤拷(x锟斤拷)锟斤拷锟斤拷,锟斤拷锟叫伙拷锟斤拷谋锟斤拷_(d锟斤拷)锟絲锟統(c锟斤拷)
锟斤拷锟斤拷锟侥獻(xi锟斤拷n)
1.Kluiver J, Gibcus JH, Hettinga C, Adema A, Richter MKS, et al. (2012) Rapid Generation of MicroRNA Sponges for MicroRNA Inhibition. PLoS ONE 7(1): e29275. doi:10.1371/journal.pone.0029275
2.Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X. (2012) Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs. Methods 58(2):118-25.
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