miRNA锟缴光定锟斤拷PCR锟斤拷驗技锟絞(sh锟斤拷)锟斤拷锟斤拷(w锟斤拷)
Real-time miRNA锟斤拷小锟侥非撅拷锟絘RNAs,锟斤拷通锟絕锟絰锟斤拷锟皆碉拷锟絚锟斤拷锟絘锟斤拷mRNA锟斤拷3' UTL锟斤拷锟叫結(ji锟斤拷)锟较匡拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟剿斤拷幕锟斤拷锟斤拷锟絖锟斤拷锟斤拷30%锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷小RNA锟斤拷锟接碉拷锟絳(di锟斤拷o)锟斤拷,锟斤拷锟侥讹拷锟絳(di锟斤拷o)锟斤拷(ji锟斤拷)锟斤拷锟絅锟斤拷锟斤功锟斤拷,锟斤拷锟斤拷锟斤拷锟絣(f锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟街伙拷,锟斤拷锟斤拷殖,锟斤拷锟斤拷锟斤拷锟斤拷锟絊锟斤拷stemness锟斤拷impriting,锟斤拷Northern blot锟角凤拷锟斤拷锟斤拷锟絅miRNAs锟斤拷锟矫的凤拷锟斤拷,锟斤拷锟斤拷Northern blot锟斤拷锟斤拷锟斤拷锟絗锟斤拷锟饺低★拷通锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷要锟斤拷锟斤拷,锟斤拷锟斤拷锟絩锟斤拷锟斤拷PCR锟斤拷锟斤拷锟斤拷锟絗锟斤拷锟饺革拷,锟斤拷锟斤拷锟斤拷锟斤拷俪蔀锟絲锟統锟斤拷一锟斤拷锟斤拷锟絜锟絰锟斤拷
锟斤拷锟节筹拷锟斤拷锟絤iRNA锟紿锟斤拷21-23nt,锟斤拷锟斤拷锟斤拷通锟絕锟斤拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟組锟叫發(f锟斤拷)锟紻(zhu锟斤拷n)锟,锟斤拷锟組锟斤拷锟組锟叫讹拷锟斤拷锟斤拷锟斤拷锟絑锟斤拷锟絖锟斤拷锟斤拷miRNA锟斤拷尾锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絤iRNA,锟斤拷然锟斤拷锟組锟斤拷miRNA锟斤拷锟斤拷锟斤拷見锟铰圖锟斤拷,锟斤拷
miRNA锟缴光定锟斤拷PCR锟斤拷锟斤拷(w锟斤拷)要锟斤拷
锟斤拷锟结供锟斤拷锟絩锟斤拷锟竭憋拷锟斤拷锟斤拷玫募锟斤拷锟斤拷锟斤拷锟斤拷锟5锟斤拷10锟斤拷6锟轿凤拷锟斤拷锟斤拷锟組锟斤拷(100mg),锟斤拷血液(300锟斤拷l)锟饺樣憋拷锟斤拷锟斤拷,锟斤拷锟津純伙拷锟矫的匡拷RNA锟斤拷OD260/OD280锟斤拷1.9-2.1之锟絞锟斤拷锟斤拷锟斤拷锟皆好o拷,锟斤拷
同锟絩锟结供锟斤拷锟統miRNA锟斤拷锟叫伙拷锟斤拷號,锟斤拷
miRNA锟缴光定锟斤拷PCR锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷(n锟斤拷i)锟斤拷
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