|
miRNA锟缴光定锟斤拷PCR锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(w锟斤拷)
Real-time miRNA锟斤拷小锟侥非撅拷锟絘RNAs锟斤拷通锟絕(gu锟斤拷)锟絰锟斤拷锟皆碉拷锟絚锟斤拷锟絘(b锟斤拷)锟斤拷mRNA锟斤拷3' UTL锟斤拷锟叫結(ji锟斤拷)锟较匡拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟剿斤拷幕锟斤拷锟斤拷锟絖(d锟斤拷),锟斤拷锟斤拷30%锟斤拷锟斤拷锟(d锟斤拷ng)锟斤拷锟斤拷锟斤拷锟叫NA锟斤拷锟接碉拷锟絳(di锟斤拷o)锟斤拷,锟斤拷锟侥讹拷锟絳(di锟斤拷o)锟斤拷(ji锟斤拷)锟斤拷锟絅锟斤拷锟斤功锟杰ｏ拷锟斤拷锟斤拷锟絣(f锟斤拷)锟斤拷,锟斤拷锟斤拷(x锟斤拷)锟斤拷锟街伙拷,锟斤拷锟斤拷殖锟斤拷锟斤拷锟斤拷,锟斤拷锟絊锟斤拷stemness锟斤拷impriting,锟斤拷Northern blot锟角凤拷锟斤拷锟斤拷锟絅miRNAs锟斤拷锟矫的凤拷锟斤拷锟斤拷锟斤拷Northern blot锟斤拷锟斤拷锟斤拷锟絗锟斤拷锟饺碉拷,锟斤拷通锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷要锟斤拷锟斤拷,锟斤拷锟斤拷(sh锟斤拷)锟絩(sh锟斤拷)锟斤拷锟斤拷PCR锟斤拷锟斤拷锟斤拷锟絗锟斤拷锟饺高★拷锟斤拷锟斤拷锟斤拷俪蔀锟絲锟統(c锟斤拷)锟斤拷一锟斤拷(g锟斤拷)锟斤拷锟絜(cu锟斤拷)锟絰锟斤拷,锟斤拷
锟斤拷锟节筹拷锟斤拷锟絤iRNA锟紿锟斤拷21-23nt,锟斤拷锟斤拷锟斤拷通锟絕(gu锟斤拷)锟斤拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟組(j锟斤拷n)锟叫發(f锟斤拷)锟紻(zhu锟斤拷n)錄ｏ拷锟組(j锟斤拷n)锟斤拷锟組(j锟斤拷n)锟叫讹拷锟斤拷,锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷锟斤拷miRNA锟斤拷尾锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)锟絤iRNA,锟斤拷然锟斤拷锟組(j锟斤拷n)锟斤拷miRNA锟斤拷锟斤拷锟斤拷見(ji锟斤拷n)锟铰圖锟斤拷锟斤拷
miRNA锟缴光定锟斤拷PCR锟斤拷锟斤拷(w锟斤拷)要锟斤拷
锟斤拷锟结供锟斤拷锟絩锟斤拷锟竭憋拷锟斤拷锟斤拷玫募锟(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷5锟斤拷10锟斤拷6锟轿凤拷锟斤拷,锟斤拷锟組锟斤拷(100mg),锟斤拷血液(300锟斤拷l)锟饺樣憋拷锟斤拷锟较ｏ拷锟津純伙拷锟矫的匡拷RNA锟斤拷OD260/OD280锟斤拷1.9-2.1之锟絞,锟斤拷锟斤拷锟斤拷锟皆好ｏ拷,锟斤拷
同锟絩(sh锟斤拷)锟结供锟斤拷锟統(c锟斤拷)miRNA锟斤拷锟叫伙拷锟斤拷號(h锟斤拷o)锟斤拷
miRNA锟缴光定锟斤拷PCR锟斤拷锟斤拷(w锟斤拷)锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
miRNA锟斤拷锟斤拷RNA锟斤拷锟斤拷锟,锟斤拷锟接撅拷D片,锟斤拷锟経(ku锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟桔斤拷锟斤拷锟斤拷,锟斤拷Ct值锟皆硷拷 锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟統(t锟斤拷ng)計(j锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟絰锟斤拷,锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷锟皆硷拷剩锟斤拷锟斤拷锟斤拷锟侥ｏ拷澹拷锟斤拷Z锟斤拷锟絖(d锟斤拷)锟缴憋拷锟斤拷1锟斤拷(g锟斤拷)锟铰ｏ拷
|
