|
LncRNA 锟統锟斤拷
锟絃鏈非撅拷锟絘RNA (long non-coding RNA) 锟斤拷一锟斤拷L锟饺达拷锟斤拷 200nt,锟斤拷锟揭诧拷锟斤拷锟絘锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷 RNA,锟斤拷锟絍锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絯锟斤拷(n锟斤拷i)锟斤拷Lnc-RNA 锟統锟斤拷锟斤拷锟饺ワ拷锟 rRNA 锟侥斤拷锟届方锟斤拷,锟斤拷锟斤拷锟斤拷锟叫革拷通锟斤拷锟統锟斤拷锟絞(sh锟斤拷)锟斤拷一锟斤拷锟皆獲锟矫單堿锟斤拷锟街憋拷锟绞碉拷锟截讹拷锟組锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷锟截讹拷锟紸锟轿碉拷锟斤拷锟斤拷 mRNA 锟斤拷lncRNA,锟斤拷锟叫撅拷 lncRNA 锟斤拷锟紻(zhu锟斤拷n)锟斤拷约锟斤拷D(zhu锟斤拷n)錄猴拷确锟斤拷鎸︼拷锟斤拷锟斤拷锟絖(d锟斤拷)锟斤拷锟絳(di锟斤拷o)锟斤拷锟斤拷锟斤拷,锟斤拷
锟斤拷锟絞(sh锟斤拷)路锟斤拷
锟酵樣斤拷锟絟
| 锟斤拷锟(Qubit) |
锟絯锟絜 |
锟斤拷锟斤拷 |
锟斤拷锟斤拷M锟斤拷锟斤拷锟斤拷 |
| ≥200ng/μL |
≥25μL |
≥5μg |
RNA 锟給锟斤拷锟斤拷, 锟給锟斤拷锟斤拷M锟斤拷染锟斤拷锟給锟斤拷锟阶猴拷锟絪锟絴(zh锟斤拷)锟斤拷染,OD260/280≥1.8,锟斤拷23S/16S≥1,RIN≥7 |
锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(sh锟斤拷)
| LncRNA 锟統锟斤拷 |
锟統锟斤拷锟斤拷锟 |
锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷 |
锟斤拷锟斤拷 |
| |
HiSeq4000 PE150 |
6~10Gb data |
45锟斤拷锟斤拷然锟斤拷 |
|
