|
植锟斤拷miRNA锟絕锟斤拷锟絖/锟斤拷锟狡憋拷锟絖锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷
MicroRNA (miRNA) 锟斤拷一锟斤拷蓛锟(n锟斤拷i)源锟斤拷锟津編碼锟斤拷锟絃锟饺約锟斤拷22 锟斤拷锟斤拷锟斤拷锟斤拷姆蔷锟斤拷a锟斤拷锟絉NA锟斤拷锟接ｏ拷锟斤拷锟斤拷锟节勶拷植锟斤拷锟叫咃拷锟絚锟紻(zhu锟斤拷n)錄猴拷锟斤拷锟斤拷锟絖锟絳(di锟斤拷o)锟斤拷,锟斤拷miRNA锟斤拷锟斤拷锟狡伙拷锟絕锟斤拷锟絖锟斤拷锟斤拷锟叫撅拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷要锟斤拷锟斤拷锟絰,锟斤拷
miRNA锟斤拷锟狡憋拷锟絖锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷
目前,锟斤拷锟斤拷要锟斤拷锟矫兩種锟街讹拷锟絖锟斤拷锟斤拷锟斤拷目锟斤拷RNA锟斤拷锟絖锟斤拷锟斤拷缘锟揭拷螅藘煞N锟斤拷锟絞(sh锟斤拷)锟窖斤拷(j锟斤拷ng)锟节勶拷植锟斤拷玫锟斤拷锟(y锟斤拷ng)锟斤拷,锟斤拷
1. 锟斤拷锟絎(xu锟斤拷)锟较成碉拷miRNA inhibitor,锟斤拷锟斤拷锟斤拷锟斤拷锟絳(di锟斤拷o)目锟侥硷拷锟斤拷锟叫碉拷miRNA 锟斤拷锟皆岋拷锟紽(xi锟斤拷n)loss-of function锟叫撅拷,锟斤拷
2. 锟斤拷锟斤拷miRNA target mimicry 锟斤拷锟絞(sh锟斤拷),锟斤拷锟絆(sh锟斤拷)計目锟剿可結(ji锟斤拷)锟斤拷miRNA锟斤拷锟給锟斤拷锟斤拷miRNA锟斤拷RISC锟叫革拷锟絫arget mimicry 锟剿癸拷锟斤拷锟斤拷锟絛锟絯锟斤拷锟斤拷锟斤拷目锟斤拷miRNA锟侥伙拷锟皆ｏ拷锟斤拷锟絤iRNA锟斤拷锟狡碉拷效锟斤拷,锟斤拷
  miRNA锟絕锟斤拷锟絖锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷
- 锟絕锟斤拷锟絖miRNA
atmiRNA,锟斤拷锟斤拷锟絕锟斤拷锟絖锟斤拷然pri-miRNA锟斤拷锟斤拷锟斤拷(j锟斤拷)锟斤拷锟斤拷锟絰锟今啟勶拷锟接硷拷锟結锟絰锟斤拷記,锟斤拷
锟斤拷锟斤拷锟侥獻
Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X. (2012) Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs. Methods 58(2):118-25.
|
