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锟斤拷(x锟斤拷)锟斤拷16S rDNA锟斤拷锟絅锟絙锟斤拷
通锟絕DNA锟統(c锟斤拷)锟斤拷?q锟斤拷)锟轿拷锟斤拷锟斤拷M(j锟斤拷n)锟斤拷锟斤拷N锟絙锟斤拷锟斤拷锟斤拷锟絅锟絙锟斤拷锟斤拷锟角比傦拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟絙锟斤拷锟斤拷锟斤拷锟組(j锟斤拷n)锟斤拷锟絙锟斤拷锟斤拷锟斤拷,锟斤拷DNA锟統(c锟斤拷)锟斤拷锟斤拷賴锟斤拷锟絅锟斤拷锟斤拷锟斤拷锟絚(di锟斤拷n)锟斤拷锟斤拷(du锟斤拷)锟斤拷锟叫撅拷锟絅锟斤拷锟斤拷使锟斤拷,锟斤拷锟饺傦拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟絙锟斤拷锟斤拷锟接匡拷锟斤拷,锟斤拷锟斤拷(zh锟斤拷n)锟絖锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷司锟结供锟斤拷微锟斤拷锟斤拷锟斤拷N锟絙锟斤拷锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷(x锟斤拷)锟斤拷16srDNA锟絙锟斤拷锟斤拷锟斤拷锟絀TS锟絙锟斤拷,锟斤拷通锟絕PCR锟経(ku锟斤拷)锟斤拷16SrDNA,锟斤拷锟統(c锟斤拷)锟斤拷锟斤拷锟紾eneBank锟斤拷锟斤拷Eztaxon锟斤拷(du锟斤拷)锟饺硷拷锟缴★拷一锟斤拷锟斤拷锟斤拷锟斤拷锟狡讹拷锟斤拷97%锟斤拷锟较就匡拷锟斤拷锟絁(r锟斤拷n)锟斤拷锟斤拷同锟絅锟斤拷锟絅,锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)通锟絕锟斤拷锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(y锟斤拷u)锟斤拷锟斤拷通锟斤拷锟斤拷锟斤拷锟絤锟斤拷锟斤拷99%锟斤拷锟较碉拷微锟斤拷锟斤拷,锟斤拷锟絙锟斤拷锟缴癸拷锟斤拷锟斤拷98%锟斤拷锟较★拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1.锟斤拷品锟斤拷式锟斤拷锟斤拷锟斤拷锟斤拷锟絩锟斤拷液,锟斤拷斜锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷,锟斤拷平锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷取锟矫碉拷DNA锟斤拷
2.锟斤拷锟斤拷锟狡凤拷锟斤拷锟斤拷MDNA,锟斤拷DNA锟斤拷锟>10ng/ul,锟斤拷锟絯锟絜锟斤拷锟斤拷20ul锟斤拷
3.锟絙锟斤拷锟結(ji锟斤拷)锟斤拷一锟斤拷锟斤拷锟斤拷b锟斤拷锟斤拷锟絅,锟斤拷锟斤拷(g锟斤拷)锟絜锟斤拷锟絅锟斤拷锟斤拷微锟斤拷锟斤數(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷锟絇(gu锟斤拷n)锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)缺锟斤拷,锟斤拷只锟斤拷锟絙锟斤拷锟斤拷锟劫★拷锟斤拷锟斤拷锟紷锟絅锟斤拷r,锟斤拷锟斤拷锟斤拷锟斤拷锟組(f锟斤拷i),锟斤拷
4.锟斤拷锟斤拷y(c锟斤拷)锟斤拷Y(ji锟斤拷)锟斤拷锟斤拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟阶峰，視锟斤拷锟狡凤拷锟斤拷锟,锟斤拷锟斤拷锟斤拷司锟斤拷锟斤拷取全锟絶锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟組(f锟斤拷i)锟斤拷,锟斤拷锟斤拷锟斤拷锟絟锟斤拷隆锟斤拷锟斤拷(g锟斤拷)锟統(c锟斤拷)锟斤拷锟絙锟斤拷锟斤拷
5.锟斤拷(du锟斤拷)锟斤拷某些锟铰诧拷锟皆撅拷锟絯,锟斤拷請(q锟斤拷ng)?zh锟斤拷)锟角帮拷f锟斤拷,锟斤拷注锟解保锟給(h锟斤拷)锟斤拷锟斤拷锟剿員锟斤拷锟斤拷锟斤拷锟絟(hu锟斤拷n)锟斤拷锟絣(w锟斤拷i)锟斤拷锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟組(j锟斤拷n)锟斤拷锟斤拷锟斤拷锟絆(sh锟斤拷)計(j锟斤拷)锟较筹拷,锟斤拷
2.PCR锟経(ku锟斤拷)锟斤拷16S rDNA,锟斤拷
3.PCR锟絘(ch锟斤拷n)锟斤純锟斤拷锟斤拷
4.锟統(c锟斤拷)锟斤拷,锟斤拷锟斤拷锟叫比岋拷(du锟斤拷)锟斤拷锟斤拷锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷锟斤拷锟絇CR锟絘(ch锟斤拷n)锟斤拷锟斤拷片,锟斤拷NCBI锟斤拷锟叫比岋拷(du锟斤拷)锟絙锟斤拷锟結(ji锟斤拷)锟斤拷,锟斤拷锟統(c锟斤拷)锟斤拷锟(b锟斤拷o)锟芥、锟斤拷锟叫堿锟斤拷锟紻,锟斤拷
注锟斤拷锟斤拷(du锟斤拷)锟斤拷锟絙锟斤拷锟侥撅拷锟斤拷,锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤拷菁锟斤拷锟斤拷锟,锟斤拷請(q锟斤拷ng)锟酵檢之前锟斤拷锟矫憋拷锟絅锟斤拷锟斤拷锟斤拷
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