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GST pull-down
GST pull-down锟斤拷锟(y锟斤拷n)锟紺锟缴傦拷(g锟斤拷)锟斤拷锟斤拷锟絯锟斤拷Y(ji锟斤拷)锟较的凤拷锟斤拷之一,锟斤拷原锟斤拷锟角ｏ拷锟斤拷锟叫碉拷锟斤拷锟絚GST锟节合憋拷锟絖(d锟斤拷),锟斤拷锟斤拷锟斤拷锟斤拷锟阶固伙拷锟节癸拷锟阶革拷锟斤拷(GSH)锟紿锟酵橈拷(sh锟斤拷)脂锟较ｏ拷锟斤拷锟斤拷锟絚目锟侥碉拷锟斤拷锟紿锟酵碉拷支锟斤拷锟斤拷,锟斤拷锟戒當(d锟斤拷ng)锟斤拷锟絋锟紻锟斤拷锟阶★拷锟斤拷目锟侥碉拷锟斤拷锟斤拷液锟絕(gu锟斤拷)锟斤拷,锟斤拷锟缴忥拷锟叫诧拷锟紷锟絚之锟洁互锟斤拷锟矫的★拷锟斤拷锟紷锟斤拷锟阶★拷锟斤拷目锟侥碉拷锟阶ｏ拷,锟斤拷洗脫锟結(ji锟斤拷)锟斤拷锟斤拷锟酵拷^(gu锟斤拷)WB锟斤拷锟斤拷锟斤拷锟侥讹拷锟紺锟斤拷(sh锟斤拷)锟缴種锟斤拷锟斤拷锟絞锟斤拷锟洁互锟斤拷锟斤拷,锟斤拷
"锟絋锟紻锟斤拷锟斤拷"锟斤拷"锟斤拷锟紷锟斤拷锟斤拷"锟斤拷锟斤拷通锟絕(gu锟斤拷)锟斤拷(x锟斤拷)锟斤拷锟窖斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟侥碉拷锟阶★拷锟斤拷锟絖(d锟斤拷)系锟統(t锟斤拷ng)锟皆硷拷锟絯锟斤拷锟紻(zhu锟斤拷n)錄凤拷锟絞系锟統(t锟斤拷ng)锟饺凤拷锟斤拷锟紷锟斤拷,锟斤拷锟剿凤拷锟斤拷锟斤拷(ji锟斤拷n)锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟姐。示锟斤拷D锟斤拷锟斤拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟絖(d锟斤拷)锟斤拷锟斤拷锟叫碉拷锟斤拷锟絚锟斤拷锟絇(gu锟斤拷n)锟絋锟紻锟斤拷锟阶ｏ拷锟斤拷锟结供锟斤拷锟斤拷锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷(g锟斤拷u)锟斤拷锟斤拷,锟斤拷锟斤拷锟竭猴拷锟斤拷锟絋锟紻锟斤拷锟阶的硷拷(x锟斤拷)锟斤拷锟窖斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷Z锟斤拷锟絖(d锟斤拷)锟斤拷司锟斤拷锟絖(d锟斤拷)锟窖解）,锟斤拷锟絲锟統(c锟斤拷)锟斤拷一锟斤拷锟斤拷GST/His/Flag锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷司锟斤拷锟組(f锟斤拷i)锟结供锟斤拷锟斤拷锟斤拷锟斤拷峁╋拷锟斤拷_(d锟斤拷)锟斤拷锟阶橈拷品锟斤拷-20锟斤拷锟斤拷妫拷锟斤拷锟斤拷\(y锟斤拷n)輸,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟斤拷(g锟斤拷u)锟斤拷原锟剿憋拷锟絖(d锟斤拷)锟絛锟絯,锟斤拷
2.锟絋锟斤拷(d锟斤拷o)锟斤拷锟阶憋拷锟絖(d锟斤拷)锟斤拷
3.GST pull-down,锟斤拷
4.SDS-PAGE 锟斤拷WB锟絲锟統(c锟斤拷),锟斤拷
5.锟斤拷锟巾實(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟芥。
锟斤拷锟斤拷(w锟斤拷)锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
锟结供锟斤拷锟斤拷锟侥岋拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷,锟斤拷 SDS-PAGE 锟斤拷WB锟結(ji锟斤拷)锟斤拷,锟斤拷 |
