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Southern Blot
Southern印锟紼锟絪锟斤拷锟斤拷Southern blot锟斤拷锟斤拷1975锟斤拷锟斤拷英锟斤拷锟斤拷southern锟斤拷(chu锟斤拷ng)锟斤拷,锟斤拷锟斤拷锟叫撅拷DNA锟紻锟絍锟侥伙拷锟斤拷锟斤拷锟絞(sh锟斤拷),锟斤拷Southern印锟紼锟絪锟斤拷锟斤拷Southern blot锟斤拷锟斤拷锟組锟叫伙拷锟斤拷MDNA锟截讹拷锟斤拷锟叫讹拷位锟斤拷通锟矫凤拷锟斤拷锟斤拷一锟斤拷锟斤拷锟矫拷脂锟斤拷锟斤拷锟絲锟接撅拷锟斤拷x锟斤拷(j锟斤拷ng)锟斤拷锟斤拷锟皆冿拷(n锟斤拷i)锟斤拷酶锟斤拷锟斤拷锟斤拷DNA片锟斤拷,锟斤拷锟斤拷锟絲锟较碉拷DNA變锟皆诧拷锟斤拷原位锟斤拷锟斤拷锟紻NA片锟斤拷锟紻(zhu锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷膜锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷支锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷(j锟斤拷ng)锟缴匡拷锟斤拷锟斤拷锟斤拷锟解線锟斤拷锟斤拷潭锟斤拷锟斤拷锟斤拷c锟斤拷锟斤拷锟斤拷(y锟斤拷ng)锟結(ji锟斤拷)锟斤拷(g锟斤拷u)锟侥橈拷記探锟斤拷M锟斤拷锟絪锟斤拷,锟斤拷锟矫凤拷锟斤拷锟斤拷锟紷影锟斤拷酶锟斤拷锟斤拷(y锟斤拷ng)锟紷色,锟斤拷锟侥讹拷锟絲锟統锟截讹拷DNA锟斤拷锟接的猴拷锟斤拷锟斤拷锟斤拷锟絑锟斤拷锟絖锟结供DIG锟斤拷記锟斤拷Southern Blot锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(w锟斤拷),锟斤拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1,锟斤拷锟斤拷锟絪锟斤拷目锟剿伙拷锟斤拷锟斤拷锟叫和猴拷锟叫达拷锟絪锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤拷(j锟斤拷)Southern blot锟斤拷锟揭拷锟斤拷锟斤拷锟斤拷x锟斤拷全锟絃锟津部分區(q锟斤拷)锟斤拷锟斤拷锟斤拷探锟斤拷锟斤拷锟�,锟斤拷使锟斤拷PCR锟斤拷锟斤拷記锟截革拷锟斤拷探锟�,锟斤拷
2锟斤拷锟斤拷锟絪锟斤拷锟侥革拷锟絴(zh锟斤拷)锟斤拷DNA锟斤拷品锟絴(zh锟斤拷)锟斤拷锟斤拷锟絇(gu锟斤拷n)锟斤拷要,锟斤拷锟絈锟斤拷锟斤拷锟侥成旓拷,锟斤拷請锟絖锟斤拷DNA锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷锟斤拷锟組锟斤拷锟斤拷锟斤拷锟組锟斤拷試锟斤拷锟斤拷锟斤拷取锟斤拷委锟斤拷锟斤拷锟絑锟斤拷锟絖锟斤拷取,锟斤拷
A,锟斤拷锟斤拷锟斤拷MDNA锟絲锟統锟斤拷OD260/OD280=1.8-2.0锟斤拷锟斤拷却锟斤拷锟�0.3锟斤拷g/ul,锟斤拷锟絪锟斤拷锟斤拷模锟斤拷DNA锟絪60 锟斤拷g/泳锟斤拷/锟斤拷,锟斤拷
B锟斤拷請?zh锟斤拷)峁╋拷锟斤拷锟組DNA锟絲锟統锟紻片,锟斤拷要锟斤拷o锟斤拷锟斤拷,锟斤拷锟給锟斤拷锟斤拷锟斤拷染锟斤拷
C,锟斤拷锟絲锟統锟斤拷品锟酵滐拷锟絓輸锟斤拷锟斤拷司,锟斤拷
3锟斤拷锟酵戯拷锟斤拷锟斤拷o锟斤拷锟结供锟斤拷品锟絴(zh锟斤拷)锟侥伙拷锟斤拷MDNA锟斤拷品,锟斤拷锟斤拷锟絑锟斤拷锟絖锟斤拷锟皆庯拷锟斤拷锟狡傦拷锟斤拷锟斤拷锟絊outhern Blot锟絲锟統锟斤拷DNA锟斤拷品,锟斤拷锟酵戯拷锟结供锟斤拷品要锟斤拷锟斤拷锟铰ｏ拷
A,锟斤拷锟斤拷液锟斤拷锟斤拷锟斤拷锟斤拷500ul锟斤拷锟斤拷锟斤拷锟斤拷锟竭干憋拷锟絓輸
B,锟斤拷锟組锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷小锟斤拷2g,锟缴憋拷锟絓輸
C锟斤拷锟斤拷锟斤拷锟斤拷锟结供锟斤拷锟絩锟侥硷拷锟斤拷锟斤拷锟斤拷,锟斤拷锟組锟斤拷锟斤拷要使锟斤拷锟絵锟斤拷液锟絖洗
4,锟斤拷請注锟斤拷锟斤拷锟斤拷MDNA酶锟叫凤拷锟斤拷锟斤拷锟斤拷锟斤拷目锟斤拷锟斤拷要锟矫碉拷锟角筹拷規(gu锟斤拷)锟斤拷锟斤拷锟斤拷锟皆冿拷(n锟斤拷i)锟斤拷酶,锟斤拷锟斤拷锟絶锟斤拷锟斤拷锟矫革拷锟斤拷M锟矫伙拷锟叫客戯拷锟结供锟斤拷锟斤拷(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟皆冿拷(n锟斤拷i)锟斤拷酶,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟斤拷锟斤拷MDNA锟斤拷取锟斤拷锟斤拷锟斤拷锟揭拷锟�,锟斤拷
2.探锟斤拷O(sh锟斤拷)計锟较成ｏ拷锟斤拷锟斤拷锟揭拷锟�,锟斤拷
3. DIG探針橈拷記锟斤拷
4. Southern锟絪锟斤拷锟絲锟統,锟斤拷
5. 锟结交锟斤拷影锟絲锟統锟斤拷锟�,锟斤拷锟斤拷锟斤拷詳锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷蛯锟斤拷Y(ji锟斤拷)锟斤拷锟饺★拷
Southern Blot锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟侥硷拷
锟斤拷锟絑锟斤拷锟絖負責锟斤拷锟酵戯拷锟结供锟斤拷品锟組锟斤拷DIG探針橈拷記锟絚Southern锟絪锟斤拷锟絲锟統锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(w锟斤拷),锟斤拷锟斤拷K锟结供锟絲锟統锟斤拷婕帮拷z锟統锟紻片
1,锟斤拷DNA锟斤拷品锟絲锟統锟斤拷妫拷锟斤拷锟狡凤拷锟斤拷锟竭€锟斤拷請注锟解保锟斤拷锟捷ｏ拷
2,锟斤拷锟剿準岋拷锟斤拷锟斤拷虉锟斤拷
3,锟斤拷southern blot锟斤拷锟結(ji锟斤拷)锟斤拷锟絲锟統锟斤拷锟�
4锟斤拷锟斤拷锟斤拷菍锟斤拷锟斤拷锟絶锟斤拷锟揭拷锟�,锟斤拷請?zh锟斤拷)锟角帮拷锟诫娀锟斤拷]锟斤拷锟絝锟斤拷
锟斤拷驗范锟斤拷锟紻片锟斤拷
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