|
16S rDNA锟侥庫構(g锟斤拷u)锟斤拷锟斤拷锟斤拷(x锟斤拷)锟斤拷群锟斤拷锟斤拷锟斤拷苑锟斤拷锟
锟斤拷(x锟斤拷)锟斤拷16S rDNA锟角硷拷(x锟斤拷)锟斤拷染色锟絯锟较撅拷锟絘16S rRNA锟斤拷DNA锟斤拷锟叫ｏ拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫硷拷(x锟斤拷)锟斤拷染色锟絯锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟杰夛拷锟紷示锟斤拷(x锟斤拷)锟斤拷锟絞锟斤拷同锟斤拷類等硷拷水平锟较碉拷锟截拷锟斤拷,锟斤拷锟絤锟斤拷锟节硷拷(x锟斤拷)锟斤拷锟紿锟斤拷锟絇(gu锟斤拷n)系锟斤拷锟叫撅拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷y锟斤拷锟斤拷(x锟斤拷)锟斤拷锟組(j锟斤拷n)锟斤拷锟斤拷锟紿锟斤拷锟絇(gu锟斤拷n)系锟斤拷锟斤拷锟矫癸拷锟斤拷,锟斤拷同锟絩锟斤拷锟斤拷锟斤拷锟矫匡拷變锟絕(q锟斤拷)锟斤拷锟叫的差異锟斤拷锟斤拷同锟斤拷锟斤拷,锟斤拷锟斤拷锟絅锟侥硷拷(x锟斤拷)锟斤拷锟組(j锟斤拷n)锟叫凤拷锟斤拷b锟斤拷锟斤拷通锟絕锟斤拷(g锟斤拷u)锟斤拷锟斤拷(x锟斤拷)锟斤拷16S rDNA锟侥庯拷,锟斤拷锟斤拷(x锟斤拷)锟斤拷16S rDNA锟斤拷锟叫碉拷DGGE變锟斤拷锟捷讹拷锟斤拷锟絲锟接撅拷锟斤拷g(sh锟斤拷)锟斤拷RFLP锟斤拷锟斤拷锟斤拷片锟斤拷锟絃锟饺讹拷锟紹(t锟斤拷i)锟皆硷拷锟絞(sh锟斤拷)锟斤拷锟斤拷锟皆岋拷某一锟截讹拷锟絟(hu锟斤拷n)锟斤拷锟叫硷拷(x锟斤拷)锟斤拷群锟斤拷Y(ji锟斤拷)锟斤拷(g锟斤拷u)锟酵硷拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟組(j锟斤拷n)锟叫凤拷锟斤拷,锟斤拷
锟斤拷(x锟斤拷)锟斤拷16S rDNA锟侥庫構(g锟斤拷u)锟斤拷
使锟斤拷PCR锟斤拷锟斤拷锟経(ku锟斤拷)锟斤拷锟截讹拷锟絟(hu锟斤拷n)锟斤拷锟斤拷品锟叫伙拷霞锟(x锟斤拷)锟斤拷16S rDNA锟斤拷使锟斤拷TA锟斤拷隆锟斤拷锟斤拷锟斤拷(g锟斤拷u)锟斤拷锟斤拷(x锟斤拷)锟斤拷16S rDNA锟侥庯拷,锟斤拷锟斤拷锟絰锟斤拷钥锟铰★拷y锟斤拷锟斤拷(x锟斤拷)锟斤拷16S rDNA锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟絚GenBank锟斤拷锟斤拷知锟斤拷锟斤拷锟組(j锟斤拷n)锟斤拷同源锟皆憋拷锟絕锟斤拷锟紷锟斤拷锟截讹拷锟絟(hu锟斤拷n)锟斤拷锟叫的硷拷(x锟斤拷)锟斤拷群锟斤拷Y(ji锟斤拷)锟斤拷(g锟斤拷u)锟酵硷拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷息,锟斤拷
RFLP锟斤拷锟斤拷锟斤拷片锟斤拷锟絃锟饺讹拷锟紹(t锟斤拷i)锟斤拷
RFLP(Restriction Fragment Length Polymorphism,锟斤拷锟斤拷片锟斤拷锟絃锟饺讹拷锟紹(t锟斤拷i)锟斤拷),锟斤拷锟斤拷锟斤拷锟截拷锟斤拷锟斤拷锟斤對锟斤拷锟轿拷锟斤拷锟饺猴拷wDNA锟斤拷取锟斤拷锟組(j锟斤拷n)锟斤拷PCR锟経(ku锟斤拷)锟斤拷锟斤拷然锟斤拷锟斤拷锟絤锟斤拷(d锟斤拷ng)?sh锟斤拷)锟斤拷锟斤拷锟斤拷詢?n锟斤拷i)锟斤拷酶锟斤拷锟経(ku锟斤拷)锟斤拷锟絘(ch锟斤拷n)锟斤拷锟組(j锟斤拷n)锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟叫的差異,锟斤拷锟绞現(xi锟斤拷n)酶锟叫圖锟絍锟斤拷 DNA锟斤拷锟紹(t锟斤拷i)锟皆★拷該锟斤拷锟斤拷锟窖憋拷锟絍锟斤拷锟斤拷锟节伙拷锟斤拷M锟絲锟斤拷锟紻锟絍锟斤拷(g锟斤拷u)锟斤拷,锟斤拷锟斤拷锟斤拷位锟皆硷拷锟斤拷锟斤拷锟組(j锟斤拷n)锟斤拷锟酵凤拷類碉拷锟叫撅拷,锟斤拷T-RFLP锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(j锟斤拷)锟斤拷(x锟斤拷)锟斤拷16S rRNA锟斤拷锟斤拷锟截區(q锟斤拷)锟絆(sh锟斤拷)計(j锟斤拷)通锟斤拷锟斤拷锟斤，锟皆达拷锟斤拷锟斤拷锟斤拷品DNA锟斤拷模锟斤拷锟組(j锟斤拷n)锟斤拷PCR锟経(ku锟斤拷)锟斤拷,锟斤拷锟斤拷锟矫碉拷锟斤拷PCR锟絘(ch锟斤拷n)锟斤拷锟斤拷锟斤拷囟锟斤拷牡锟斤拷锟斤拷锟斤拷詢锟(n锟斤拷i)锟斤拷酶锟組(j锟斤拷n)锟斤拷酶锟斤拷,锟斤拷锟斤拷锟斤拷锟节硷拷(x锟斤拷)锟斤拷之锟絞16S rRNA锟斤拷锟斤拷锟叫猴拷锟斤拷锟斤拷锟斤拷锟叫的差異锟斤拷酶锟叫猴拷锟斤拷a(ch锟斤拷n)锟斤拷锟杰多不同锟絃锟饺碉拷锟斤拷锟斤拷锟斤拷片锟斤拷,锟斤拷通锟絕锟絲锟統锟斤拷锟斤拷锟紷些酶锟斤拷片锟轿达拷小锟斤拷锟缴凤拷锟斤拷(y锟斤拷ng)微锟斤拷锟斤拷群锟斤拷M锟斤拷锟斤拷r,锟斤拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1.請?zh锟斤拷)峁╋拷锟斤拷锟組DNA锟斤拷锟斤拷(j锟斤拷ng)锟斤拷锟絅锟斤拷锟絰锟斤拷锟叫單撅拷锟斤拷锟斤拷锟斤拷r平锟斤拷锟叫憋拷锟,锟斤拷
2.锟斤拷锟斤拷MDNA锟侥濓拷取锟10 ng/锟斤拷l,锟斤拷锟斤拷锟絯锟絜锟斤拷20 锟斤拷l锟斤拷锟揭無锟斤拷锟紷锟斤拷锟斤拷,锟斤拷平锟斤拷锟叫憋拷鎽(y锟斤拷ng)锟絃锟叫凤拷锟絰锟侥單撅拷锟斤拷,锟斤拷
3.锟斤拷锟节革拷锟絤锟斤拷锟斤拷约锟(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟脚撅拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟,锟斤拷請?ji锟斤拷n)诜锟斤拷?w锟斤拷)委锟叫曪拷锟叫硷拷锟斤拷锟絝锟斤拷,锟斤拷
4.锟絟(hu锟斤拷n)锟斤拷锟斤拷品锟斤拷锟斤拷锟叫的硷拷(x锟斤拷)锟斤拷锟角凤拷锟斤拷锟斤拷虏锟斤拷院蜐锟斤拷锟轿ｏ拷U(xi锟斤拷n),锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷品锟斤拷锟斤拷一锟斤拷锟斤拷锟铰诧拷锟皆伙拷锟斤拷袧锟斤拷诘锟轿ｏ拷U(xi锟斤拷n)锟斤拷請锟斤拷锟结供锟接憋拷DNA,锟斤拷
5.請锟斤拷锟结供锟絰锟斤拷些锟斤拷锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟組(j锟斤拷n)锟叫凤拷锟斤拷,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
锟揭傦拷锟结供锟給锟斤拷DNA锟接撅拷z锟統锟紻锟斤拷锟斤拷y锟斤拷颖锟斤拷臏y锟斤拷锟絟锟紻,锟斤拷锟斤拷锟叫比岋拷锟結(ji锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟侥結(ji锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷(b锟斤拷o)锟斤拷,锟斤拷
|
