|
锟斤拷锟斤拷芯片锟絲锟統锟斤拷PCR Array锟斤拷
PCR Array锟街憋拷锟絈锟介功锟杰凤拷锟叫酒拷锟絇CR锟斤拷锟,锟斤拷锟結锟斤拷qPCR锟斤拷锟絞锟絗锟斤拷锟缴匡拷锟皆硷拷微锟斤拷屑锟斤拷g同锟絩锟絲锟統锟斤拷N锟斤拷锟斤拷锟斤拷_锟斤拷锟侥冿拷(y锟斤拷u)锟捷★拷锟斤拷锟斤拷锟斤拷號通路锟斤拷某锟斤拷锟斤拷W锟斤拷锟斤拷锟斤拷锟絇锟斤拷锟斤拷锟斤拷_锟斤拷B(t锟斤拷i)锟斤拷****锟斤拷锟斤拷,锟斤拷
锟斤拷锟斤拷要锟斤拷
1. 锟絖锟絁锟斤拷號通路锟斤拷锟斤拷N锟斤拷息,锟斤拷
2. 锟结供锟斤拷锟絩锟津保达拷锟斤拷玫募锟斤拷锟斤拷锟 锟斤拷锟斤拷5锟斤拷10锟斤拷6锟轿凤拷锟斤拷锟斤拷锟組锟斤拷(锟斤拷锟斤拷50mg),锟斤拷血液(300锟斤拷l)锟饺樣憋拷锟斤拷锟斤拷,锟斤拷锟津純伙拷锟矫的匡拷RNA锟斤拷OD260/OD280锟斤拷1.9-2.1之 锟絞锟斤拷锟斤拷锟斤拷锟皆好ｏ拷锟斤拷DNA锟斤拷OD260/OD280锟斤拷1.7-1.9之锟絞,锟斤拷锟斤拷锟斤拷锟皆好ｏ拷,锟斤拷锟斤拷锟紻錄好碉拷cDNA锟斤拷锟斤拷锟斤拷30锟斤拷l锟斤拷锟斤拷锟紻錄的匡拷RNA锟斤拷锟斤拷锟皆好ｏ拷锟斤拷锟斤拷锟斤拷
1.9-2.1之锟絞锟斤拷,锟斤拷
锟斤拷锟秸伙拷锟斤拷锟斤拷锟斤拷
1.锟斤拷品RNA锟斤拷锟,锟斤拷锟
2.RNA锟絴(zh锟斤拷)锟斤拷锟絲锟統,锟斤拷锟絲锟統RNA锟斤拷锟饺硷拷锟斤拷锟斤拷锟斤拷,锟斤拷锟结供RNA锟絴(zh锟斤拷)锟斤拷锟斤拷妫
3.cDNA锟较筹拷,锟斤拷使锟斤拷锟斤拷锟紻锟矫,锟斤拷锟斤拷锟斤拷品RNA锟斤拷锟紻錄烇拷cDNA锟斤拷
4.锟斤拷锟絩锟斤拷锟斤拷PCR锟斤拷锟斤拷,锟斤拷
5.锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷,锟斤拷計锟斤拷PCR芯片锟叫的革拷锟斤拷Ct值锟斤拷锟斤拷锟矫︼拷锟斤拷Ct锟斤拷锟斤拷锟斤拷锟絕锟斤拷锟斤拷谋锟斤拷_變锟斤拷,锟斤拷
锟斤拷锟絇锟紹锟接ｏ拷
锟缴光定锟斤拷PCR
miRNA锟缴光定锟斤拷PCR
lncRNA锟缴光定锟斤拷PCR
circRNA锟缴光定锟斤拷PCR
锟斤拷螌锟斤拷晒舛拷锟絇CR锟斤拷qPCR锟斤拷锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟組锟叫統(t锟斤拷ng)計锟斤拷锟斤拷
锟缴光定锟斤拷PCR锟斤拷qPCR锟斤拷锟斤拷锟絞锟斤拷锟絵
|
