锟缴光定锟斤拷PCR锟斤拷qPCR锟斤拷锟斤拷锟斤拷锟斤拷PCR锟経锟斤拷锟絕锟斤拷锟斤拷,锟斤拷通锟絕锟缴癸拷锟斤拷號,锟斤拷锟斤拷PCR锟組锟斤拷锟組锟叫岋拷锟絩锟絲锟統锟斤拷锟斤拷锟斤拷锟斤拷PCR锟経锟斤拷锟斤拷指锟斤拷(sh锟斤拷)锟絩锟斤拷,锟斤拷模锟斤拷锟紺t值锟斤拷該模锟斤拷锟斤拷锟绞硷拷锟截愶拷锟(sh锟斤拷)锟斤拷锟节撅拷锟斤拷锟絇系,锟斤拷锟斤拷锟皆成為定锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟节筹拷規(gu锟斤拷)锟斤拷PCR锟斤拷缺锟絚,锟斤拷
锟缴光定锟斤拷PCR锟斤拷 qPCR锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷悖拷`锟斤拷锟饺革拷,锟斤拷锟截忥拷锟皆好等冿拷(y锟斤拷u)锟絚锟絣(f锟斤拷)展锟角筹拷迅锟斤拷,锟斤拷锟絆锟斤拷锟窖斤拷锟芥及锟斤拷锟斤拷锟斤拷锟狡學锟叫撅拷锟侥革拷锟斤拷锟絀锟津，憋拷锟斤拷锟斤拷锟侥差異锟斤拷锟絖锟斤拷锟斤拷,锟斤拷SNP锟絲锟統,锟斤拷锟斤拷位锟斤拷锟斤拷臋z锟統锟斤拷藥锟斤拷锟絖锟絣(f锟斤拷),锟斤拷锟絉锟斤拷锟絓锟斤拷,锟斤拷锟紻锟斤拷锟斤拷锟叫撅拷锟饺★拷
锟斤拷锟斤拷锟斤拷锟絕
锟缴光定锟斤拷PCR锟斤拷qPCR锟斤拷锟斤拷锟叫撅拷锟斤拷锟斤拷锟絕锟侥儀锟斤拷也锟給锟街猴拷ABI 7000,锟斤拷7300,锟斤拷7500锟斤拷7700,锟斤拷7900HT,锟斤拷StepOnePlus TM锟斤拷StepOne TM,锟斤拷PRISM? StepOne TM系锟斤拷,锟斤拷BIO-RAD锟斤拷CFX96锟斤拷iCycler iQ5?,锟斤拷MyiQ?,锟斤拷MJ Research Chromo4 TM Opticon系锟叫ｏ拷Stratagene Mx TM系锟斤拷,锟斤拷Roche LightCycler?系锟斤拷,锟斤拷Eppendorf Masercycler?锟斤拷Corbett Rotor-Gene TM,锟斤拷Cepheid SmartCycler? 锟斤拷BIOER 锟斤拷LineGene系锟斤拷,锟斤拷锟斤拷x锟斤拷锟斤拷锟絆锟矫和旓拷(sh锟斤拷)锟斤拷輸锟斤拷锟斤拷式锟斤拷锟斤拷锟斤拷疲锟斤拷锟筋不锟斤拷,锟斤拷
锟斤拷锟絕qPCR锟斤拷驗經锟斤拷锟叫撅拷锟竭匡拷锟杰很匡拷锟斤拷锟斤拷锟较わ拷洳煌拷锟PCR锟侥結锟斤拷Ct值,锟斤拷锟皆硷拷锟斤拷(sh锟斤拷)锟斤拷锟侥筹拷锟斤拷锟絓锟姐方锟斤拷锟斤拷锟絕Ct锟斤拷锟斤拷锟斤拷2-锟斤拷锟斤拷Ct锟斤拷锟斤拷锟斤拷么锟紷锟斤拷锟斤拷(sh锟斤拷)锟斤拷锟斤拷锟斤拷锟叫沒锟叫統(t锟斤拷ng)計锟斤拷锟絰锟斤拷,锟斤拷锟紷锟斤拷該锟斤拷畏锟斤拷锟,锟斤拷锟揭傦拷锟斤拷锟斤拷u锟剿斤拷锟斤拷锟斤拷锟斤拷锟斤拷qPCR锟侥旓拷(sh锟斤拷)锟斤拷锟統(t锟斤拷ng)計锟斤拷锟斤拷,锟斤拷
锟斤拷锟斤拷锟节旓拷(sh锟斤拷)锟斤拷锟斤拷锟斤拷之前锟斤拷要锟絖锟斤拷锟斤拷(sh锟斤拷)锟斤拷锟侥準確锟斤拷,锟斤拷也锟斤拷锟角岋拷锟斤拷^锟教的革拷锟斤拷锟斤拷锟絋锟斤拷要锟斤拷锟斤拷锟斤拷锟,锟斤拷也锟斤拷锟斤拷
MIQE锟剿準ｏ拷锟斤拷锟絯锟斤拷見Bustin SA et al Clin Chem 55,611-622 2009锟斤拷锟斤拷锟紷些锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷么锟酵匡拷锟皆侊拷锟斤拷锟斤拷Ct锟斤拷(sh锟斤拷)锟斤拷锟斤拷,锟斤拷
锟統(t锟斤拷ng)計锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷3锟斤拷锟斤拷(sh锟斤拷)锟斤拷锟脚匡拷锟斤拷,锟斤拷锟角撅拷锟斤拷锟斤拷锟絆計锟斤拷锟斤拷锟,锟斤拷每锟組锟斤拷锟斤拷要锟斤拷3锟斤拷锟斤拷锟斤拷W锟截復ｏ拷也锟斤拷锟斤拷3锟斤拷锟斤拷同锟侥樣憋拷锟斤拷锟斤拷锟紷锟斤拷锟斤拷qPCR锟斤拷3锟斤拷PCR replicate锟斤拷锟斤拷全锟斤拷同锟侥革拷锟筋（锟紷锟斤拷锟斤拷同一锟斤拷锟接憋拷锟斤拷3锟斤拷锟截復ｏ拷,锟斤拷
锟斤拷锟斤拷锟揭傦拷锟斤拷锟斤拷一锟斤拷處锟斤拷锟斤拷锟斤拷锟侥岋拷锟斤拷锟斤拷锟絝锟斤拷锟斤拷蝸頂(sh锟斤拷)锟斤拷锟統(t锟斤拷ng)計锟斤拷锟斤拷,锟斤拷1锟斤拷處锟斤拷锟組锟斤拷1锟斤拷锟斤拷锟秸組锟斤拷每锟組锟斤拷3锟斤拷锟接憋拷锟斤拷也锟斤拷锟斤拷锟斤拷锟斤拷W锟截復ｏ拷,锟斤拷锟斤拷z锟統某目锟侥伙拷锟斤拷target,锟斤拷锟絆锟斤拷锟饺咃拷锟斤拷锟斤拷锟絩eference锟斤拷锟斤拷锟斤拷锟紷锟斤拷處锟斤拷锟斤拷,锟斤拷目锟侥伙拷锟斤拷谋锟斤拷_锟角凤拷锟杰碉拷锟斤拷锟斤拷,锟斤拷同锟絩锟紷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟角凤拷锟叫統(t锟斤拷ng)計锟絎锟斤拷锟絰锟斤拷
锟斤拷锟斤拷锟斤拷锟侥傦拷qPCR锟絰,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟剿檢锟統,锟斤拷锟斤拷(sh锟斤拷)锟斤拷也锟斤拷锟斤拷锟斤拷锟斤拷excel锟斤拷w锟絳锟斤拷锟铰ｏ拷
Group |
Sample |
Ct target |
Ct mean of T |
Ct reference |
Ct mean of R |
CON |
sample1 |
22.89 |
22.90 |
15.29 |
15.63 |
22.82 |
15.91 |
23.00 |
15.69 |
sample2 |
22.60 |
22.64 |
15.83 |
15.60 |
22.46 |
15.71 |
22.85 |
15.27 |
sample3 |
22.49 |
22.53 |
14.81 |
14.98 |
22.49 |
14.79 |
22.60 |
15.34 |
TRT |
sample1 |
25.32 |
25.31 |
14.98 |
15.18 |
25.40 |
15.33 |
25.20 |
15.22 |
sample2 |
25.70 |
25.60 |
15.46 |
15.38 |
25.50 |
15.45 |
25.61 |
15.22 |
sample3 |
25.49 |
25.60 |
15.25 |
15.05 |
25.68 |
14.6 |
25.64 |
15.29 |
锟斤拷锟斤拷每锟斤拷锟斤拷品锟斤拷目锟侥伙拷锟斤拷蛢葏锟斤拷锟斤拷锟斤拷3锟斤拷PCR replicate要锟斤拷锟狡斤拷锟街碉拷锟斤拷@锟斤拷锟紿锟紿锟角硷拷锟絞锟截忥拷,锟斤拷也锟斤拷锟角確锟斤拷锟紷锟斤拷锟斤拷(sh锟斤拷)锟斤拷锟角可匡拷锟斤拷,锟斤拷然锟斤拷锟斤拷锟斤拷锟矫匡拷锟斤拷锟狡凤拷锟侥匡拷幕锟斤拷锟斤拷變锟斤拷锟斤拷也锟斤拷锟角ｏ拷目锟侥伙拷锟斤拷锟斤拷锟斤拷锟斤拷鋵︼拷锟斤拷膬葏锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷龋锟揭诧拷锟斤拷锟2-锟斤拷Ct锟斤拷,锟斤拷然锟斤拷锟斤拷平锟斤拷锟斤拷锟秸組锟叫碉拷3锟斤拷2-锟斤拷Ct,锟斤拷锟节达拷锟斤拷锟斤拷锟叫旓拷(sh锟斤拷)锟斤拷锟斤拷0.006482锟斤拷然锟斤拷锟斤拷锟斤拷2-锟斤拷Ct锟侥讹拷锟斤拷0.006482锟斤拷燃锟斤拷傻锟2-锟斤拷锟斤拷Ct,锟斤拷也锟斤拷锟角憋拷锟斤拷(sh锟斤拷)變锟斤拷锟斤拷fold change锟斤拷锟斤拷relative expression锟斤拷,锟斤拷
锟斤拷锟斤拷涂锟斤拷詫锟2-锟斤拷锟斤拷Ct锟斤拷锟斤拷2-锟斤拷Ct锟斤拷锟竭撅拷锟叫統(t锟斤拷ng)計锟斤拷锟斤拷锟斤拷Ct锟斤拷2锟絅锟斤拷式锟斤拷锟斤拷锟斤拷锟組锟叫統(t锟斤拷ng)計锟斤拷锟斤拷,锟斤拷唯锟斤拷锟斤拷锟杰岋拷Ct锟組锟叫統(t锟斤拷ng)計锟斤拷锟斤拷,锟斤拷锟斤拷锟絯锟統(t锟斤拷ng)計锟斤拷锟斤拷锟侥凤拷锟斤拷锟斤拷锟轿何統(t锟斤拷ng)計锟斤拷锟斤拷軟锟斤拷锟斤拷锟斤拷锟斤拷锟矫憋拷锟斤拷SPSS,锟斤拷GraphPad锟斤拷,锟斤拷锟紷锟斤拷展示一锟斤拷GraphPad锟斤拷锟斤拷锟侥結锟斤拷锟斤拷
Group |
Sample |
Ct target |
Ct mean of T |
Ct reference |
Ct mean of R |
2-锟斤拷Ct |
2-锟斤拷锟斤拷Ct |
CON |
sample1 |
22.89 |
22.90 |
15.29 |
15.63 |
0.006464 |
0.99724 |
22.82 |
15.91 |
23.00 |
15.69 |
sample2 |
22.60 |
22.64 |
15.83 |
15.60 |
0.007634 |
1.17773 |
22.46 |
15.71 |
22.85 |
15.27 |
sample3 |
22.49 |
22.53 |
14.81 |
14.98 |
0.005348 |
0.82512 |
22.49 |
14.79 |
22.60 |
15.34 |
TRT |
sample1 |
25.32 |
25.31 |
14.98 |
15.18 |
0.000892 |
0.13768 |
25.40 |
15.33 |
25.20 |
15.22 |
sample2 |
25.70 |
25.60 |
15.46 |
15.38 |
0.000835 |
0.12875 |
25.50 |
15.45 |
25.61 |
15.22 |
sample3 |
25.49 |
25.60 |
15.25 |
15.05 |
0.000664 |
0.10243 |
25.68 |
14.6 |
25.64 |
15.29 |
锟斤拷锟絇锟紹锟接ｏ拷
锟缴光定锟斤拷PCR
miRNA锟缴光定锟斤拷PCR
lncRNA锟缴光定锟斤拷PCR
circRNA锟缴光定锟斤拷PCR
锟缴光定锟斤拷PCR锟斤拷qPCR锟斤拷锟斤拷锟絞锟斤拷锟絵
