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锟絟(hu锟斤拷n)锟斤拷RNA锟斤拷circRNA锟斤拷锟缴光定锟斤拷PCR(qPCR)
circRNA锟斤拷Circular RNA,锟斤拷锟絟(hu锟斤拷n)锟斤拷RNA锟斤拷锟接ｏ拷锟斤拷一類诧拷锟斤拷锟斤拷5' 末锟斤拷帽锟接猴拷3' 末锟斤拷poly(A)尾锟酵★拷锟斤拷锟皆癸拷锟絩(ji锟斤拷)锟絀锟轿成環(hu锟斤拷n)锟轿結(ji锟斤拷)锟斤拷(g锟斤拷u)锟侥非撅拷锟絘RNA锟斤拷锟斤拷,锟斤拷目前锟絣(f锟斤拷)锟紽(xi锟斤拷n)锟斤拷circRNA锟斤拷要锟斤拷源锟节伙拷锟斤拷锟斤拷锟紷锟斤拷,锟斤拷锟斤拷锟斤拷锟(xi锟斤拷ng)锟叫撅拷也锟斤拷锟斤拷circRNA锟斤拷锟斤拷捅锟斤拷锟斤拷锟斤拷要锟斤拷(f锟斤拷)锟絪锟斤拷锟斤拷锟皆侊拷锟皆伙拷锟斤拷M锟较讹拷N锟斤拷锟斤拷Y(ji锟斤拷)锟斤拷(g锟斤拷u),锟斤拷 锟絤然circRNA锟斤拷規(gu锟斤拷)模锟叫撅拷锟絩(sh锟斤拷)锟絞锟斤拷锟絃(zh锟斤拷ng),锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷(j锟斤拷)锟斤拷锟斤拷锟斤拷m(x锟斤拷)锟斤拷CNS锟斤拷锟节匡拷锟较發(f锟斤拷)锟斤拷锟斤拷锟叫撅拷锟结潮锟斤拷然锟斤拷然锟斤拷锟斤拷,锟斤拷 锟斤拷锟节結(ji锟斤拷)锟斤拷(g锟斤拷u)锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟紷也锟給锟叫撅拷circRNA锟斤拷刹锟斤拷俚锟斤拷锟斤拷y锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絆(sh锟斤拷)計(j锟斤拷)锟斤拷锟斤拷锟斤拷一,锟斤拷
锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟斤拷锟斤拷锟斤拷锟絆(sh锟斤拷)計(j锟斤拷)锟侥時(sh锟斤拷)锟斤拷,锟斤拷锟絰锟斤拷锟节環(hu锟斤拷n)锟斤拷位锟絚(di锟斤拷n)锟斤拷锟斤拷锟斤拷锟絆(sh锟斤拷)計(j锟斤拷)一锟斤拷(du锟斤拷)锟斤拷U(ku锟斤拷)锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟解對(du锟斤拷)锟斤拷锟皆擴(ku锟斤拷)锟斤拷,锟斤拷锟斤拷锟斤拷锟截拷锟皆★拷  锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1.锟结供锟斤拷锟統(c锟斤拷)锟斤拷锟斤拷锟斤拷锟叫伙拷锟斤拷號(h锟斤拷o),锟斤拷
2.锟结供锟斤拷锟絩锟津保达拷锟斤拷玫募锟(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷5锟斤拷10锟斤拷6锟轿凤拷锟斤拷,锟斤拷锟組锟斤拷(锟斤拷锟斤拷50mg)锟斤拷血液(300锟斤拷l)锟饺樣憋拷锟斤拷锟较ｏ拷锟津純伙拷锟矫的匡拷RNA
锟斤拷OD260/OD280锟斤拷 1.9-2.1之锟絞,锟斤拷锟斤拷锟斤拷锟皆好ｏ拷锟斤拷DNA锟斤拷OD260/OD280锟斤拷1.7-1.9之锟絞,锟斤拷锟斤拷锟斤拷锟皆好ｏ拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄好碉拷cDNA锟斤拷锟斤拷锟斤拷
30锟斤拷l锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄的匡拷RNA锟斤拷锟斤拷 锟皆猴拷,锟斤拷锟斤拷锟斤拷锟斤拷1.9-2.1之锟絞锟斤拷,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟结供锟斤拷要锟絲锟統(c锟斤拷)锟斤拷circRNA锟斤拷锟斤拷锟斤拷锟絈锟斤拷锟斤拷锟教(h锟斤拷o)锟斤拷锟斤拷锟斤拷,锟斤拷
2.锟絖锟絁(r锟斤拷n)锟接憋拷锟斤拷(sh锟斤拷)锟斤拷锟斤拷锟絅锟斤拷,锟斤拷锟接憋拷锟斤拷锟,锟斤拷
3.锟斤拷訂锟斤拷同锟斤拷支锟斤拷锟紸(y锟斤拷)锟斤拷锟斤拷,锟斤拷
4.锟结供锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷品锟斤拷锟斤拷(x锟斤拷)锟斤拷,锟斤拷锟組锟斤拷锟斤拷血液锟斤拷RNA/cDNA锟斤拷,锟斤拷
5.锟絖展锟斤拷(sh锟斤拷)锟(y锟斤拷n),锟斤拷
6.锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟結(ji锟斤拷)锟斤拷锟襟，發(f锟斤拷)锟酵筹拷锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷,锟斤拷
7.支锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)尾锟斤拷,锟斤拷
8.锟絣(f锟斤拷)锟斤拷詳锟斤拷(x锟斤拷)锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟芥，锟斤拷蓪锟(sh锟斤拷)锟(y锟斤拷n)锟(xi锟斤拷ng)目,锟斤拷
锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
詳锟斤拷(x锟斤拷)锟侥岋拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟紼,锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷試锟斤拷,锟斤拷锟絰锟斤拷,锟斤拷锟斤拷(bi锟斤拷o)锟斤拷(zh锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷,锟斤拷
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