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锟斤拷锟斤拷
锟絃鏈非撅拷锟絘RNA(long noncoding RNA, lncRNA)指锟斤拷锟斤拷锟絃锟饺筹拷锟絕200nt锟斤拷RNA锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷锟斤拷,锟斤拷位锟节硷拷锟斤拷锟剿伙拷锟斤拷|锟斤拷(n锟斤拷i)锟斤拷锟斤拷锟叫憋拷锟截的讹拷锟斤拷锟結锟斤拷,锟斤拷锟斤拷锟絚锟斤拷锟斤拷锟絴,锟斤拷DNA锟斤拷RNA锟洁互锟斤拷锟矫ｏ拷通锟絕锟斤拷N锟紺锟狡ｏ拷锟斤拷锟斤拷锟接∮,锟斤拷染色锟絴锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絳(di锟斤拷o)锟截★拷锟斤拷锟斤拷锟絳(di锟斤拷o)锟斤拷,锟斤拷mRNA锟斤拷锟斤拷头锟斤拷g锟絳(di锟斤拷o)锟截等ｏ拷锟节讹拷N锟斤拷锟斤拷锟较ｏ拷锟斤拷锟斤拷^锟絲锟斤拷锟絎,锟斤拷锟紻锟斤拷{(di锟斤拷o)锟截硷拷锟紻錄猴拷锟絳(di锟斤拷o)锟截等ｏ拷锟絳(di锟斤拷o)锟截伙拷锟斤拷谋锟斤拷_水平锟斤拷
lncRNA锟斤拷锟絚锟斤拷N锟斤拷锟斤拷W锟絕锟教碉拷锟絳(di锟斤拷o)锟截ｏ拷锟节讹拷N锟斤拷锟斤拷锟斤拷锟斤拷邪l(f锟斤拷)锟絔锟斤拷要锟斤拷锟斤拷,锟斤拷該锟絀锟斤拷锟斤拷P注锟斤拷也锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟窖成烇拷锟絕锟斤拷使RNA(mRNA)锟斤拷小RNA(microRNA,锟斤拷siRNA锟斤拷)之锟斤拷锟杰伙拷锟斤拷M锟絎锟角,锟斤拷锟斤拷锟斤拷芯锟斤拷I锟斤拷
锟斤拷锟斤拷要锟斤拷
1.锟结供锟斤拷锟統锟斤拷锟斤拷锟斤拷锟叫伙拷锟斤拷號,锟斤拷
2.锟结供锟斤拷锟絩锟津保达拷锟斤拷玫募锟斤拷锟斤拷锟锟斤拷锟斤拷5锟斤拷10锟斤拷6锟轿凤拷锟斤拷,锟斤拷锟組锟斤拷(锟斤拷锟斤拷50mg)锟斤拷血液(300锟斤拷l)锟饺樣憋拷锟斤拷锟斤拷,锟斤拷锟津純伙拷锟矫的匡拷RNA
锟斤拷OD260/OD280锟斤拷 1.9-2.1之锟絞,锟斤拷锟斤拷锟斤拷锟皆好ｏ拷锟斤拷DNA锟斤拷OD260/OD280锟斤拷1.7-1.9之锟絞锟斤拷锟斤拷锟斤拷锟皆好ｏ拷,锟斤拷锟斤拷锟紻錄好碉拷cDNA锟斤拷锟斤拷锟斤拷30锟斤拷l锟斤拷锟斤拷锟紻錄的匡拷RNA锟斤拷锟斤拷 锟皆猴拷,锟斤拷锟斤拷锟斤拷锟斤拷1.9-2.1之锟絞锟斤拷锟斤拷
锟斤拷锟秸伙拷锟斤拷锟斤拷锟斤拷
1.锟结供锟斤拷要锟絲锟統锟斤拷lncRNA锟斤拷锟斤拷锟斤拷锟絈,锟斤拷锟斤拷锟教栵拷锟斤拷锟斤拷校锟
2.锟絖锟絁锟接憋拷锟斤拷(sh锟斤拷)锟斤拷,锟斤拷锟絅锟斤拷,锟斤拷锟接憋拷锟斤拷停锟
3.锟斤拷訂锟斤拷同,锟斤拷支锟斤拷锟紸锟斤拷锟斤拷,锟斤拷
4.锟结供锟斤拷锟斤拷品锟斤拷锟斤拷锟斤拷锟斤拷锟組锟斤拷,锟斤拷血液锟斤拷RNA/cDNA锟斤拷,锟斤拷
5.锟絖展锟斤拷驗；
6.锟斤拷锟結锟斤拷锟斤拷,锟斤拷锟絣(f锟斤拷)锟酵筹拷锟斤拷锟斤拷锟斤拷锟,锟斤拷
7.支锟斤拷锟斤拷锟轿诧拷睿
8.锟絣(f锟斤拷)锟斤拷詳锟斤拷锟斤拷锟斤拷锟,锟斤拷锟斤拷蓪锟斤拷锟侥,锟斤拷
锟斤拷锟斤拷(n锟斤拷i)锟斤拷
詳锟斤拷锟侥岋拷驗步锟紼锟斤拷锟斤拷驗方锟斤拷,锟斤拷锟斤拷锟斤拷試锟斤拷,锟斤拷锟絰锟斤拷锟斤拷锟剿滐拷锟斤拷锟斤拷,锟斤拷锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷,锟斤拷
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