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锟斤拷锟18S rDNA/ITS锟斤拷锟絅锟絙锟斤拷
锟斤拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷(l锟斤拷i)锟絙锟斤拷锟斤拷要锟斤拷锟斤拷(j锟斤拷)锟斤拷锟轿態(t锟斤拷i),锟斤拷锟斤拷锟絃(zh锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷變锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷然锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷N锟(l锟斤拷i)锟斤拷,锟斤拷锟斤拷锟斤拷锟絃(zh锟斤拷ng)锟轿態(t锟斤拷i),锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷變锟斤拷锟斤拷锟絊锟絟(hu锟斤拷n)锟斤拷锟斤拷變锟斤拷也锟斤拷锟斤拷(w锟斤拷n)锟斤拷锟斤拷锟斤拷锟,锟斤拷锟斤拷锟矫傦拷锟統(t锟斤拷ng)锟斤拷锟斤拷锟絙锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟,锟斤拷锟絊锟斤拷锟斤拷锟斤拷锟斤技锟絞(sh锟斤拷)锟絣(f锟斤拷)展锟斤拷DNA锟斤拷锟斤拷锟窖斤拷(j锟斤拷ng)锟絍锟斤拷锟統(c锟斤拷)锟斤拷,锟斤拷通锟絕(gu锟斤拷)锟斤拷(du锟斤拷)锟斤拷锟斤拷锟斤拷锟阶冿拷锟斤拷锟斤拷袦y(c锟斤拷)锟斤拷,锟斤拷锟斤拷锟組(j锟斤拷n)锟叫撅拷锟絅锟絙锟斤拷锟斤拷目前锟斤拷锟矫碉拷锟斤拷18S rDNA锟斤拷ITS,锟斤拷通锟絕(gu锟斤拷)PCR锟経(ku锟斤拷)锟斤拷,锟斤拷锟統(c锟斤拷)锟斤拷锟絚GenBank锟叫碉拷锟斤拷知锟斤拷锟斤拷锟組(j锟斤拷n)锟叫比岋拷(du锟斤拷)锟斤拷,锟斤拷锟叫讹拷锟斤拷锟斤拷N锟(l锟斤拷i),锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷值锟斤拷倩锟絅锟斤拷
锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟结供锟経(ku锟斤拷)锟斤拷锟斤拷锟18S rRNA锟斤拷锟斤拷,锟斤拷锟絪1,700 bp,锟斤拷锟斤拷ITS锟斤拷锟斤拷锟斤拷锟300-700bp,锟斤拷锟組(j锟斤拷n)锟叫擴(ku锟斤拷)锟斤拷,锟斤拷锟統(c锟斤拷)锟斤拷BLAST锟斤拷锟斤拷(du锟斤拷)锟斤拷品锟組(j锟斤拷n)锟斤拷锟絙锟斤拷,锟斤拷锟斤拷锟斤拷锟絀TS (Internal Transcribed Space) 锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷ITS1锟斤拷ITS2锟缴诧拷锟街ｏ拷锟組(j锟斤拷n)锟斤拷锟劫度匡拷,锟斤拷锟斤拷锟叫讹拷锟紹(t锟斤拷i)锟斤拷,锟斤拷锟絤锟节岋拷(du锟斤拷)锟紿锟斤拷锟絇(gu锟斤拷n)系锟絕锟斤拷锟侥撅拷锟斤拷锟組(j锟斤拷n)锟叫區(q锟斤拷)锟斤拷锟絙锟斤拷锟斤拷
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1.锟斤拷品锟斤拷式锟斤拷锟斤拷锟斤拷锟斤拷锟絩锟斤拷液,锟斤拷斜锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷,锟斤拷平锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷取锟矫碉拷DNA锟斤拷
2.锟斤拷锟斤拷锟狡凤拷锟斤拷锟斤拷MDNA,锟斤拷DNA锟斤拷锟>10ng/ul,锟斤拷锟絯锟絜锟斤拷锟斤拷20ul锟斤拷
3.锟絙锟斤拷锟結(ji锟斤拷)锟斤拷一锟斤拷锟斤拷锟斤拷b锟斤拷锟斤拷锟絅,锟斤拷锟斤拷(g锟斤拷)锟絜锟斤拷锟絅锟斤拷锟斤拷微锟斤拷锟斤數(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷(k锟斤拷)锟斤拷锟絇(gu锟斤拷n)锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)缺锟斤拷,锟斤拷只锟斤拷锟絙锟斤拷锟斤拷锟劫★拷锟斤拷锟斤拷锟紷锟絅锟斤拷r,锟斤拷锟斤拷锟斤拷锟斤拷锟組(f锟斤拷i),锟斤拷
4.锟斤拷锟斤拷y(c锟斤拷)锟斤拷Y(ji锟斤拷)锟斤拷锟斤拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟阶峰，視锟斤拷锟狡凤拷锟斤拷锟,锟斤拷锟斤拷锟斤拷司锟斤拷锟斤拷取全锟絶锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟組(f锟斤拷i)锟斤拷,锟斤拷锟斤拷锟斤拷锟絟锟斤拷隆锟斤拷锟斤拷(g锟斤拷)锟統(c锟斤拷)锟斤拷锟絙锟斤拷锟斤拷
5.锟斤拷(du锟斤拷)锟斤拷某些锟铰诧拷锟皆撅拷锟絯,锟斤拷請(q锟斤拷ng)?zh锟斤拷)锟角帮拷f(shu锟斤拷)锟斤拷,锟斤拷注锟解保锟給(h锟斤拷)锟斤拷锟斤拷锟剿員锟斤拷锟斤拷锟斤拷锟絟(hu锟斤拷n)锟斤拷锟絣(w锟斤拷i)锟斤拷锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟斤拷锟絑锟斤拷锟絖(d锟斤拷)锟組(j锟斤拷n)锟斤拷锟斤拷锟斤拷锟絆(sh锟斤拷)計(j锟斤拷)锟较筹拷,锟斤拷
2. PCR锟経(ku锟斤拷)锟斤拷18S rDNA/ITS,锟斤拷
3. PCR锟絘(ch锟斤拷n)锟斤純锟斤拷锟斤拷
4. 锟統(c锟斤拷)锟斤拷,锟斤拷锟斤拷锟叫比岋拷(du锟斤拷)锟斤拷锟斤拷,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷(b锟斤拷o)锟斤拷锟(n锟斤拷i)锟斤拷
锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷(b锟斤拷o)锟斤拷锟斤拷锟絇CR锟絘(ch锟斤拷n)锟斤拷锟斤拷片锟斤拷NCBI锟斤拷锟叫比岋拷(du锟斤拷)锟絙锟斤拷锟結(ji锟斤拷)锟斤拷,锟斤拷锟統(c锟斤拷)锟斤拷锟(b锟斤拷o)锟斤拷,锟斤拷锟斤拷锟叫堿锟斤拷锟紻锟斤拷
注锟斤拷锟斤拷(du锟斤拷)锟斤拷锟絙锟斤拷锟侥撅拷锟斤拷,锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤拷菁锟斤拷锟斤拷锟,锟斤拷請(q锟斤拷ng)锟酵檢之前锟斤拷锟矫憋拷锟絅锟斤拷锟斤拷锟斤拷
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