|
植锟斤蛋锟阶伙拷锟斤拷锟斤拷C BiFC/LCI
锟絧锟斤拷锟接熒光互锟絘(b锟斤拷) (Bimolecular Fluorescence Complementation, BiFC)锟斤拷一項匡拷直锟絕,锟斤拷锟斤拷锟斤拷锟叫旓拷目锟斤拷(bi锟斤拷o)锟斤拷锟斤拷锟节活細(x锟斤拷)锟斤拷锟叫的讹拷位锟斤拷锟洁互锟斤拷锟矫碉拷锟铰硷拷锟絞(sh锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷某些锟截讹拷锟斤拷位锟絚锟斤拷锟絖,锟斤拷锟轿成诧拷锟絣(f锟斤拷)锟缴癸拷锟絅锟斤拷C锟斤拷2锟斤拷锟斤拷锟斤拷,锟斤拷锟絈锟斤拷N片锟斤拷(N-fragment)锟斤拷C片锟斤拷(C-fragment)锟斤拷锟紷2锟斤拷片锟斤拷锟节硷拷(x锟斤拷)锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟斤拷锟絖(d锟斤拷)锟斤拷锟絯锟斤拷锟较時,锟斤拷锟斤拷锟斤拷锟皆發(f锟斤拷)锟截組锟絙锟斤拷锟斤拷锟斤拷锟侥熒光蛋锟斤拷,锟斤拷锟斤拷該锟缴光蛋锟阶的硷拷锟絣(f锟斤拷)锟解激锟絣(f锟斤拷)锟絩锟斤拷锟杰產(ch锟斤拷n)锟斤拷锟缴光。锟斤拷锟斤拷,锟斤拷锟斤拷(d锟斤拷ng)锟紷2锟斤拷锟缴光蛋锟阶碉拷片锟轿分別锟紹锟接碉拷1锟組锟斤拷锟洁互锟斤拷锟矫碉拷目锟斤拷(bi锟斤拷o)锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟侥匡拷锟(bi锟斤拷o)锟斤拷锟斤拷锟絴(zh锟斤拷)之锟絞锟斤拷锟洁互锟斤拷锟矫ｏ拷锟絫锟节硷拷锟絣(f锟斤拷)锟斤拷募锟斤拷l(f锟斤拷)锟斤拷,锟斤拷锟絘(ch锟斤拷n)锟斤拷該锟缴光蛋锟阶的熒癸拷,锟斤拷锟斤拷之锟斤拷锟斤拷锟斤拷锟斤拷锟絴(zh锟斤拷)之锟絞锟絔锟斤拷锟洁互锟斤拷锟斤拷,锟斤拷锟絫锟斤拷锟杰憋拷锟斤拷锟絣(f锟斤拷)锟絘(ch锟斤拷n)锟斤拷锟缴癸拷,锟斤拷
锟斤拷锟絞(sh锟斤拷)原锟斤拷
螢锟斤拷锟絰锟缴癸拷锟斤拷酶片锟轿伙拷锟絘(b锟斤拷)锟紻锟斤拷锟絞(sh锟斤拷)锟斤拷LCI锟斤拷锟角斤拷锟斤拷锟斤拷纬傻锟斤拷芯锟斤拷锟斤拷谆锟斤拷锟斤拷锟斤拷录锟斤拷g(sh锟斤拷)锟斤拷锟斤拷2锟斤拷目锟斤拷(bi锟斤拷o)锟斤拷锟阶分別锟紹锟斤拷锟斤拷螢锟斤拷锟絰锟缴癸拷锟斤拷酶锟斤拷C锟剿猴拷N锟剿★拷锟斤拷锟2锟斤拷锟斤拷锟阶伙拷锟斤拷,锟斤拷N锟轿猴拷C锟剿接斤拷锟轿筹拷锟叫伙拷锟皆的熒癸拷锟斤拷酶,锟斤拷锟斤拷锟节燂拷锟斤拷瞬锟絩锟斤拷锟絖(d锟斤拷)锟斤拷LCI锟斤拷锟絞(sh锟斤拷)锟斤拷LCI锟斤拷锟絞(sh锟斤拷)锟絚锟斤拷锟斤拷瞬锟絩锟斤拷锟絖(d锟斤拷)锟斤拷Y(ji锟斤拷)锟斤拷,锟斤拷锟斤拷锟叫撅拷植锟斤蛋锟阶碉拷锟洁互锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟皆岋拷锟紽(xi锟斤拷n)锟斤拷锟絯锟斤拷(x锟斤拷)锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟絩锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷植锟斤拷锟皆發(f锟斤拷)锟缴癸拷母蓴_,锟斤拷锟斤拷锟揭诧拷锟斤拷锟斤拷锟轿ｏ拷锟斤拷锟斤拷要锟窖解細(x锟斤拷)锟斤拷锟斤拷取锟斤拷锟斤拷锟絴(zh锟斤拷),锟斤拷锟斤拷锟皆很好的凤拷映植锟斤拷锟斤拷锟斤拷l锟斤拷锟铰碉拷锟阶的伙拷锟斤拷锟斤拷B(t锟斤拷i),锟斤拷
锟斤拷锟斤拷锟紻片
锟斤拷锟斤拷(w锟斤拷)要锟斤拷
1.锟斤拷锟斤拷锟斤拷锟叫伙拷(g锟斤拷u)锟斤拷锟矫碉拷锟絴(zh锟斤拷)锟斤拷锟斤拷
2.锟结供锟斤拷锟絩锟津保达拷锟斤拷玫锟街诧拷锟組锟斤拷锟斤拷RNA,锟斤拷
锟斤拷锟斤拷(w锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷
1.锟斤拷锟斤拷锟铰,锟斤拷BiFC/LCI锟絛锟絯锟斤拷(g锟斤拷u)锟斤拷锟斤拷
2.锟紻(zhu锟斤拷n)锟斤拷锟斤拷,锟斤拷
3.锟絕锟斤拷,锟斤拷
4.锟斤拷锟斤拷妗
锟斤拷锟斤拷锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷锟斤拷锟斤拷
1.锟斤拷锟斤拷锟侥岋拷锟斤拷锟,锟斤拷锟斤拷锟斤拷原始锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷),锟斤拷詳锟斤拷(x锟斤拷)锟侥岋拷驗步锟紼锟斤拷锟斤拷锟斤拷锟結(ji锟斤拷)锟斤拷锟斤拷
2.锟斤拷(g锟斤拷u)锟斤拷锟矫碉拷锟絴(zh锟斤拷)锟斤拷锟斤拷锟絩(n锟斤拷ng)锟経锟斤拷锟斤拷锟斤拷,锟斤拷
|
