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锟斤拷锟斤拷D(zhu锟斤拷n)錄組锟統锟斤拷
锟紻(zhu锟斤拷n)錄組锟斤拷锟截讹拷锟組锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷某一锟斤拷锟杰狅拷B(t锟斤拷i)锟斤拷锟斤拷锟斤拷锟紻(zhu锟斤拷n)錄憋拷锟侥匡拷锟斤拷,锟斤拷锟斤拷锟斤拷 mRNA 锟酵非撅拷锟絘 RNA锟斤拷锟斤拷Non-coding RNA锟斤拷锟斤拷 tRNA,锟斤拷rRNAs,锟斤拷microRNAs,锟斤拷piRNAs 锟斤拷 long ncRNAs 锟饺ｏ拷,锟斤拷锟紻(zhu锟斤拷n)錄組锟統锟斤拷 (Transcriptome sequencing) 锟斤拷要指通锟絕锟斤拷通锟斤拷锟統锟斤拷锟叫撅拷锟截讹拷锟組锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷某锟斤拷锟絩锟斤拷锟紻(zhu锟斤拷n)錄筹拷锟斤拷锟侥★拷mRNA,锟斤拷锟斤拷锟斤拷锟矫侊拷锟叫撅拷锟斤拷锟斤拷Y(ji锟斤拷)锟斤拷(g锟斤拷u)锟酵伙拷锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷锟紸(y锟斤拷)锟統锟斤拷锟紻(zhu锟斤拷n)錄憋拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫撅拷锟紻(zhu锟斤拷n)錄組锟斤拷(f锟斤拷)锟絪锟皆的忥拷(qi锟斤拷ng)锟斤拷锟街段和癸拷锟竭★拷目前,锟斤拷锟紻(zhu锟斤拷n)錄組锟統锟斤拷锟窖斤拷(j锟斤拷ng)锟斤拷锟絍锟斤拷锟斤拷(y锟斤拷ng)锟斤拷锟节勶拷植锟斤拷l(f锟斤拷)锟斤拷锟紸锟轿碉拷锟叫撅拷,锟斤拷锟斤拷(y锟斤拷u)锟斤拷品锟絅锟斤拷锟斤拷暮Y锟絰锟酵硷拷锟斤拷锟轿拷锟饺★拷
锟斤拷锟絞(sh锟斤拷)路锟斤拷
锟酵樣斤拷锟絟
| 锟斤拷N |
锟斤拷锟(Qubit) |
锟絯锟絜 |
锟斤拷锟斤拷 |
锟斤拷锟斤拷M锟斤拷锟斤拷锟斤拷 |
| 锟斤拷锟 total RNA |
锟斤拷20ng/锟斤拷L |
15锟斤拷L-100 锟斤拷L |
锟斤拷2锟斤拷g |
RIN锟斤拷6.5 28S/18S锟斤拷1.0;锟紻锟絍锟斤拷锟斤拷锟給锟斤拷抬,锟斤拷5S?锟斤拷锟斤拷锟斤拷
|
| 锟斤拷锟斤拷锟斤拷,锟斤拷植锟斤拷total RNA |
锟斤拷20ng/锟斤拷L |
15锟斤拷L-100 锟斤拷L |
锟斤拷2锟斤拷g |
RIN锟斤拷6.5 28S/18S锟斤拷1.0;锟紻锟絍锟斤拷锟斤拷锟給锟斤拷抬锟斤拷5S?锟斤拷锟斤拷锟斤拷
|
| 原锟斤拷 total RNA |
锟斤拷20ng/锟斤拷L |
15锟斤拷L-100 锟斤拷L |
锟斤拷2锟斤拷g |
RIN锟斤拷6.5 28S/18S锟斤拷1.0;锟紻锟絍锟斤拷锟斤拷锟給锟斤拷抬,锟斤拷5S?锟斤拷锟斤拷锟斤拷
|
锟斤拷锟絞(sh锟斤拷)锟斤拷锟斤拷(sh锟斤拷)
| 锟叫咃拷锟紻(zhu锟斤拷n)錄組锟統锟斤拷 |
锟統锟斤拷锟斤拷锟 |
锟斤拷(sh锟斤拷)锟斤拷(j锟斤拷)锟斤拷 |
锟斤拷锟斤拷 |
| |
HiSeq4000 PE150 |
6G clean date |
45锟斤拷锟斤拷然锟斤拷 |
|
