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1.Western Blot锟絲锟統锟接憋拷要锟斤拷
锟斤拷锟絩锟侥橈拷品,锟斤拷锟組锟斤拷锟斤拷植锟斤拷M锟斤拷锟斤拷锟斤拷锟斤拷M锟斤拷锟斤拷,锟斤拷锟斤拷(x锟斤拷)锟斤拷,锟斤拷锟斤拷锟斤拷锟絑锟斤拷锟絖(d锟斤拷)負(f锟斤拷)責(z锟斤拷)锟斤拷取锟斤拷锟阶★拷
锟組锟斤拷锟斤拷品锟斤拷锟结供250-500mg锟斤拷锟絩锟斤拷品/锟斤拷,锟斤拷
锟斤拷(x锟斤拷)锟斤拷锟斤拷品锟斤拷锟结供1锟斤拷106锟斤拷1锟斤拷107锟斤拷(x锟斤拷)锟斤拷/锟斤拷,锟斤拷
锟斤拷锟较橈拷品锟斤拷锟斤拷要锟缴憋拷锟絓(y锟斤拷n)輸锟斤拷
注锟斤拷锟絅锟节硷拷(x锟斤拷)锟斤拷锟斤拷處锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟较诧拷锟斤拷锟斤拷
1) 锟紻(zhu锟斤拷n)染锟斤拷募锟(x锟斤拷)锟斤拷,锟斤拷锟斤拷(x锟斤拷)锟斤拷锟斤拷直锟接癸拷锟铰侊拷,锟斤拷PBS锟截戯拷锟斤拷洗锟斤拷锟斤拷洗锟斤拷锟絰锟侥ｏ拷5000 rpm,锟斤拷4min锟斤拷去锟斤拷锟斤拷-20锟芥保锟斤拷,锟斤拷锟斤拷锟解反锟斤拷(f锟斤拷)锟斤拷锟节★拷
2) 锟窖斤拷液锟窖斤拷,锟斤拷锟窖斤拷锟斤拷x锟侥ｏ拷12000 rpm,锟斤拷4min锟斤拷锟斤拷取锟斤拷锟斤拷,锟斤拷
2.Western Blot锟絲锟統锟叫烇拷什么锟絲锟統锟給锟斤拷號,锟斤拷锟斤拷锟阶板）
锟斤拷趴锟斤拷Y(ji锟斤拷)锟斤拷一锟铰庯拷锟皆拷锟
锟斤拷1锟斤拷锟斤拷品锟斤拷目锟侥碉拷锟斤拷锟絊锟饺很低ｏ拷锟斤拷锟节岋拷(sh锟斤拷)锟(y锟斤拷n)锟侥檢锟統锟斤拷锟斤拷
锟斤拷2锟斤拷一锟斤拷锟斤拷锟絉锟絜锟絲锟統锟斤拷N锟侥碉拷锟斤拷,锟斤拷同锟絩(sh锟斤拷)锟斤拷锟斤拷锟斤拷詤锟斤拷盏锟皆掞拷峁╋拷锟皆岋拷锟秸碉拷锟斤拷,锟斤拷锟斤拷锟斤拷詫锟斤拷锟斤拷锟斤拷锟斤拷t锟絝锟斤拷锟斤拷锟絯锟斤拷锟斤拷(sh锟斤拷)锟(y锟斤拷n)锟斤拷锟秸沒锟叫嗭拷锟絵锟斤拷锟斤拷锟斤拷锟角樣憋拷锟斤拷目锟侥碉拷锟阶猴拷锟斤拷锟斤拷锟絕锟斤拷,锟斤拷
锟斤拷3锟斤拷锟斤拷锟阶斤拷锟斤拷
(4锟斤拷锟斤拷锟統锟斤拷品锟侥確锟斤拷锟斤拷锟,锟斤拷
锟斤拷5锟斤拷一锟斤拷失效锟斤拷
锟斤拷6锟斤拷锟斤拷原锟斤拷锟斤拷锟斤拷,锟斤拷每泳锟斤拷锟斤拷锟斤拷锟较橈拷锟斤拷锟斤拷锟斤拷锟斤拷0.1ug,锟斤拷
3.Western Blot锟絲锟統锟叫烇拷什么锟絰锟斤拷锟(n锟斤拷i)锟斤拷锟斤拷
锟斤拷(n锟斤拷i)锟斤拷也锟叫匡拷锟揭伙拷锟斤拷,锟斤拷锟节組锟斤拷锟斤拷(x锟斤拷)锟斤拷锟叫讹拷锟斤拷锟斤拷锟絖(d锟斤拷),锟斤拷锟揭憋拷锟絖(d锟斤拷)锟斤拷锟节诧拷同锟組锟斤拷锟斤拷(x锟斤拷)锟斤拷锟叫幾猴拷锟斤拷锟斤拷同锟侥ｏ拷一锟斤拷锟叫︼拷-Tublin,锟斤拷锟斤拷-actin,锟斤拷GAPDH锟饺ｏ拷锟斤拷Western Blot锟斤拷使锟矫冿拷(n锟斤拷i)锟斤拷锟戒實(sh锟斤拷)锟斤拷锟斤拷锟斤拷Western Blot锟絕锟斤拷锟斤拷锟矫冿拷(n锟斤拷i)锟斤拷锟斤拷锟斤拷(y锟斤拷ng)锟侥匡拷锟絯锟絲锟統锟斤拷(n锟斤拷i)锟斤拷,锟斤拷
锟斤拷锟斤拷锟斤拷锟叫兩傦拷(g锟斤拷):
锟斤拷1锟斤拷锟斤拷锟斤拷取锟斤拷锟斤拷锟絴(zh锟斤拷)锟斤拷锟侥好夛拷,锟斤拷锟斤拷锟斤拷锟絯estern锟侥時(sh锟斤拷)锟斤拷,锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟斤拷锟斤拷锟叫條锟斤拷锟斤拷锟斤拷目锟侥碉拷锟阶沒锟斤拷,锟斤拷锟絝锟斤拷锟斤拷锟阶碉拷锟斤拷取锟斤拷锟紻(zhu锟斤拷n)膜锟饺诧拷锟紼锟絔锟叫嗭拷锟絵,锟斤拷锟斤拷目锟侥碉拷锟阶的匡拷锟絯锟絴(zh锟斤拷)锟斤拷锟斤拷锟矫伙拷锟斤拷锟斤拷稀釋憋拷锟斤拷(sh锟斤拷)锟斤拷锟斤拷锟斤拷
锟斤拷2锟斤拷锟斤拷锟絕锟斤拷锟絕锟斤拷锟絝锟斤拷目锟侥碉拷锟阶的憋拷锟絖(d锟斤拷)锟斤拷r,锟斤拷锟斤拷锟斤拷锟较橈拷锟斤拷锟饺碉拷锟絗锟斤拷,锟斤拷锟斤拷同锟侥橈拷品之锟絞锟斤拷锟节碉拷锟斤拷锟斤拷取锟斤拷锟斤拷锟叫诧拷e锟斤拷锟斤拷锟杰忥拷(qi锟斤拷ng)锟斤拷缘谋锟斤拷_(d锟斤拷)锟杰筹拷锟斤拷锟侥庯拷锟斤拷锟斤拷,锟斤拷
锟斤拷锟斤拷O(sh锟斤拷)锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟斤拷,锟斤拷锟斤拷锟絕锟斤拷同锟斤拷品之锟絞锟侥憋拷锟絖(d锟斤拷)锟斤拷r锟斤拷
3.Western Blot锟絲锟統锟叫烇拷什么锟斤拷(n锟斤拷i)锟斤拷锟絣锟斤拷锟斤拷一锟斤拷,锟斤拷
锟紷锟斤拷(g锟斤拷)锟斤拷锟斤拷锟,锟斤拷F(xi锟斤拷n)锟斤拷锟斤拷論锟斤拷,锟斤拷目锟侥碉拷锟斤拷锟节岋拷锟斤拷(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟叫的憋拷锟斤拷锟斤拷锟斤拷锟斤拷同,锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟斤拷目锟侥碉拷锟阶的憋拷锟斤拷也锟斤拷锟斤拷锟斤拷(y锟斤拷ng)锟組锟斤拷锟斤拷一锟铰★拷同锟接碉拷,锟斤拷锟斤拷(n锟斤拷i)锟斤拷锟斤拷一锟铰的時(sh锟斤拷)锟斤拷,锟斤拷锟较樣匡拷锟斤拷锟斤拷锟斤拷锟斤拷同锟斤拷锟斤拷目锟侥碉拷锟阶ｏ拷锟斤拷(n锟斤拷i)锟斤拷锟斤拷锟阶ｏ拷锟节诧拷同锟斤拷品锟斤拷锟斤拷取锟斤拷膬锟(n锟斤拷i)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷一锟斤拷锟斤拷锟斤拷全一锟铰碉拷,锟斤拷锟斤拷锟斤拷锟斤拷锟組(j锟斤拷n)锟斤拷Western Blot锟斤拷锟斤拷锟斤拷锟斤拷锟絩(sh锟斤拷)锟斤拷通锟絕锟斤拷(n锟斤拷i)锟斤拷锟組(j锟斤拷n)锟斤拷校锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷K锟紷锟斤拷锟斤拷锟斤拷锟侥憋拷锟絖(d锟斤拷)锟斤拷锟侥凤拷锟斤拷锟結(ji锟斤拷)锟斤拷,锟斤拷
4.Western Blot锟絲锟統锟叫烇拷什么锟絲锟統锟侥結(ji锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷小锟絚锟斤拷(sh锟斤拷)锟紿锟斤拷锟斤拷,锟斤拷
锟斤拷1锟斤拷锟斤拷锟絞锟斤拷锟斤拷飾★拷锟斤拷锟界蛋锟阶碉拷锟斤拷锟结化锟斤拷锟角伙拷锟斤拷锟斤拷,锟斤拷锟紷些锟斤拷锟斤拷锟斤拷锟接碉拷锟阶的凤拷锟斤拷锟斤拷锟斤拷小,锟斤拷
锟斤拷2锟斤拷锟斤拷锟絞锟斤拷锟斤拷小锟斤拷锟斤拷锟杰多蛋锟斤拷锟斤拷锟饺憋拷锟较成烇拷前锟絯锟斤拷式锟斤拷然锟斤拷通锟絕锟斤拷锟叫獲锟斤拷锟斤拷锟斤拷W(xu锟斤拷)锟斤拷锟斤拷,锟斤拷
锟斤拷3锟斤拷锟斤拷锟斤拷變锟斤拷锟絯锟斤拷锟斤拷同锟侥伙拷锟斤拷,锟斤拷锟斤拷(j锟斤拷ng)锟絕锟絰锟斤拷锟皆硷拷锟接曪拷锟絘(ch锟斤拷n)锟斤拷锟斤拷同锟斤拷锟斤拷锟斤拷锟斤拷小锟侥碉拷锟阶★拷
锟斤拷4锟斤拷锟斤拷锟斤拷電荷★拷锟斤拷锟斤拷锟斤拷慕M锟斤拷 (锟斤拷锟 vs 锟斤拷锟斤拷锟),锟斤拷
锟斤拷5锟斤拷锟斤拷锟斤拷w锟斤拷锟斤拷锟界蛋锟阶讹拷锟斤拷锟絯,锟斤拷
5.锟斤拷什么锟絲锟統锟結(ji锟斤拷)锟斤拷锟叫很讹拷锟絪锟斤拷锟斤拷
(1)锟斤拷锟斤拷锟角橈拷品锟斤拷锟斤拷锟絯锟斤拷(n锟斤拷i)锟斤拷锟絖(d锟斤拷)锟侥碉拷锟阶撅拷锟叫讹拷N锟斤拷锟斤拷锟绞斤拷锟斤拷腔锟斤拷锟,锟斤拷锟斤拷锟结化,锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷,锟斤拷
(2)锟斤拷锟角硷拷(x锟斤拷)锟斤拷锟斤拷品,锟斤拷锟斤拷锟斤拷锟角硷拷(x锟斤拷)锟斤拷锟斤拷锟斤拷锟轿旓拷(sh锟斤拷)锟絕锟斤拷,锟斤拷使锟戒蛋锟阶憋拷锟絖(d锟斤拷)锟斤拷同锟斤拷锟斤拷锟斤拷使锟矫傦拷锟絖(d锟斤拷)锟轿旓拷(sh锟斤拷)锟劫碉拷锟組(j锟斤拷n)锟斤拷平锟叫岋拷(sh锟斤拷)锟(y锟斤拷n)一锟斤拷锟斤拷锟斤拷锟秸匡拷锟斤拷,锟斤拷
(3)锟斤拷锟阶斤拷锟斤拷,锟斤拷锟斤拷(d锟斤拷o)锟铰碉拷锟阶凤拷锟斤拷锟斤拷锟斤拷锟酵ｏ拷
(4)锟斤拷锟斤拷锟轿成讹拷锟斤拷w锟斤拷式,锟斤拷
6.锟斤拷什么Western Blot锟絲锟統锟侥憋拷锟斤拷锟斤拷锟絕锟斤拷,锟斤拷
(1)锟斤拷锟杰匡拷锟絯锟斤拷锟斤拷^锟竭ｏ拷
(2)锟斤拷原锟斤拷锟絕锟斤拷,锟斤拷
(3)一锟斤拷膜锟斤拷锟叫碉拷目锟侥條锟斤拷锟絕锟斤拷,锟斤拷锟斤拷锟斤拷锟斤拷讓锟絕锟斤拷锟絣锟斤拷锟紷示锟斤拷锟斤拷锟斤拷锟截癸拷r(sh锟斤拷)锟絞锟絕锟絃,锟斤拷
7.锟斤拷什么Western Blot锟侥結(ji锟斤拷)锟斤拷锟絚QPCR锟結(ji锟斤拷)锟斤拷趨锟捷诧拷一锟斤拷,锟斤拷
锟紷锟絅锟斤拷r锟角憋拷锟絕锟斤拷锟斤拷锟侥ｏ拷 Western Blot锟斤拷映锟斤拷锟角碉拷锟斤拷水平锟侥結(ji锟斤拷)锟斤拷,锟斤拷 qPCR锟斤拷映锟斤拷锟角伙拷锟斤拷水平锟侥結(ji锟斤拷)锟斤拷,锟斤拷锟斤拷論锟较碉拷锟斤拷水平锟絚锟斤拷锟斤拷水平锟斤拷一锟铰的ｏ拷锟斤拷锟斤拷mRNA锟侥高低诧拷锟斤拷锟斤拷锟斤拷锟斤拷_(d锟斤拷)锟斤拷锟阶的高碉拷,锟斤拷
也锟斤拷一些锟斤拷锟斤拷锟斤拷锟斤拷诮M锟斤拷锟斤拷品锟叫沒锟斤拷锟斤拷锟斤拷锟斤拷锟絞,锟斤拷锟紷锟接硷拷使qPCR锟絲锟統锟結(ji锟斤拷)锟斤拷锟叫憋拷锟絖(d锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟阶沒锟斤拷锟斤拷锟斤拷锟斤拷锟絞锟斤拷話,锟斤拷Western Blot锟絫锟絲锟統锟斤拷锟斤拷目锟侥條锟斤拷,锟斤拷
锟斤拷锟絇(gu锟斤拷n)锟紹锟接ｏ拷
Western Blot锟絲锟統
锟缴光定锟斤拷PCR锟絲锟統
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