TRIzol 試锟斤拷锟角伙拷锟节拷锟斤拷锟斤拷锟斤拷锟斤拷/锟斤拷锟接凤拷锟斤拷一锟斤拷锟斤拷锟斤拷RNA锟斤拷取試锟斤拷,锟斤拷锟斤拷一小锟絩锟斤拷(n锟斤拷i)锟斤拷锟缴從硷拷锟斤拷锟酵組锟斤拷锟斤拷锟斤拷煽锟絉NA锟斤拷锟斤拷取,锟斤拷锟节橈拷品锟窖斤拷锟斤拷{锟絕锟斤拷锟叫o拷TRIzol試锟斤拷锟斤拷锟窖解細锟斤拷锟斤拷同锟絩,锟斤拷锟斤拷锟斤拷锟斤拷RNase锟斤拷锟斤拷,锟斤拷锟斤拷锟斤拷RNA锟斤拷锟斤拷锟皆★拷锟斤拷锟斤拷锟饺仿猴拷,锟斤拷锟斤拷液锟街烇拷水锟斤拷,锟斤拷锟斤拷锟絞锟接猴拷锟叫機锟洁,RNA锟斤拷锟斤拷锟斤拷水锟斤拷锟斤拷,锟斤拷取锟斤拷水锟斤拷,锟斤拷锟接碉拷锟絯锟絜锟斤拷锟斤拷锟斤拷锟缴筹拷锟斤拷锟斤拷RNA,锟斤拷锟斤拷锟絞锟斤拷锟斤拷锟揭达拷锟斤拷锟斤拷锟斤拷取DNA锟斤拷锟叫機锟斤拷锟矫拷锟斤拷锟斤拷锟斤拷锟斤拷锟缴伙拷锟秸碉拷锟斤拷,锟斤拷
TRIzol試锟斤拷锟絤锟矫凤拷锟斤拷锟絍,锟斤拷锟斤拷锟斤拷锟斤、植锟斤拷图锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷M锟斤拷锟斤拷取通锟斤拷,锟斤拷锟絤锟斤拷锟斤拷小锟斤拷锟斤拷品锟斤拷50-100mg锟組锟斤拷,锟斤拷5×10锟斤拷6锟轿凤拷锟斤拷锟斤拷锟斤拷锟斤拷RNA锟斤拷取锟斤拷也锟斤拷锟斤拷锟节达拷锟斤拷锟斤拷品锟斤拷锟斤拷1g 锟組锟斤拷锟斤拷10锟斤拷7锟轿凤拷锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷同锟絩處锟斤拷锟斤拷锟斤拷锟斤拷同锟斤拷品,锟斤拷RNA锟斤拷锟绞高★拷锟斤拷取锟侥匡拷RNA锟斤拷锟斤拷锟斤拷Northern Blot,锟斤拷Dot blot,锟斤拷cDNA 锟较成o拷RT-PCR,锟斤拷qPCR锟斤拷锟斤拷锟轿岋拷锟,锟斤拷
1. TRIzol Regent锟斤拷锟缴,锟斤拷锟斤拷锟斤拷水锟斤拷锟斤拷袡C锟斤拷姆直妫
2. 一锟斤拷锟斤拷锟斤拷RNA锟侥匡拷锟斤拷锟斤拷取,锟斤拷