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Thermo 反轉(zhuǎn)錄試劑盒/Thermo First Strand cDNA Synthesis Kit
貨號(hào)：Q1010 規(guī)格：20T 價(jià)格：500
貨號(hào)：Q1010L 規(guī)格：50T 價(jià)格：1050
產(chǎn)品簡(jiǎn)介
本制品包含cDNA第一鏈合成所需要的全部試劑,，其中的反轉(zhuǎn)錄酶可以利用oligo(dT)18 primer/Random primer/基因特異性引物等進(jìn)行反轉(zhuǎn)錄反應(yīng),，合成的第一鏈cDNA可廣泛用于cDNA第二鏈的合成、雜交,、PCR擴(kuò)增,、Real-Time PCR反應(yīng)等。該反轉(zhuǎn)錄酶是對(duì)M-MLV進(jìn)行定點(diǎn)突變修飾后的新型反轉(zhuǎn)錄酶,，具有較弱的RNAseH 活性和較高的熱穩(wěn)定性,，酶活性高，反轉(zhuǎn)錄效率快,�,？稍�42℃－60℃之間合成較長(zhǎng)的cDNA。試劑盒中的RNAase inhibitor對(duì)RNAase表現(xiàn)高度的非競(jìng)爭(zhēng)性抑制,，從而降低RNAase對(duì)RNA的影響,，利于反轉(zhuǎn)錄反應(yīng)的進(jìn)行。本試劑盒同時(shí)優(yōu)化了反應(yīng)Buffer,，能使反應(yīng)的模板量（Total RNA）可在0.1-5 μg范圍之間進(jìn)行逆轉(zhuǎn)錄反應(yīng),。cDNA合成后進(jìn)行PCR擴(kuò)增既可檢測(cè)到長(zhǎng)cDNA（>10 kb）片段，也可檢測(cè)到低豐度cDNA樣品,。
產(chǎn)品儲(chǔ)存條件
1. -20℃,，一年有效,；
發(fā)表文獻(xiàn)精選：
[1] IF=2.323 Ovarian Circular RNAs Associated with High and Low
Fertility in Large White Sows during the Follicular
and Luteal Phases of the Estrous Cycle;Animals 2020, 10(4), 696
[2] IF=2.985 A zebrafish (danio rerio) model for high-throughput screening food
and drugs with uric acid-lowering activity; Biochemical and Biophysical Research Communications 508 (2019) 494e498
[3] IF=3.191 Genome-Wide DNA Methylation Profile
of Gene cis-Acting Element Methylations
in All-trans Retinoic Acid-Induced Mouse Cleft Palate; DNA AND CELL BIOLOGY
Volume 37, Number 12, 2018
[4] IF=2.274 Analysis of long non-coding RNA expression profiles following focal cerebral
ischemia in mice; Neuroscience Letters 665 (2018) 123–129
[5] IF=2.773 Accumulating mechanism of γ-aminobutyric acid in soybean
(Glycine max L.) during germination; International Journal of Food Science and Technology 2018, 53, 106–111
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