2×實時熒光定量PCR Mix/2×SG Green qPCR Mix
貨號:Q1001 規(guī)格:1ml(20µl×100rxn) 價格:150
貨號:Q1001L 規(guī)格:5ml(20µl×500rxn) 價格:750
產(chǎn)品簡介
本制品是2×采用SYBR® Green I嵌合熒光法進行real-time PCR的專用預(yù)混試劑,。制品中含有real-time PCR反應(yīng)的最適濃度SYBR® Green I及****的反應(yīng)緩沖體系,Taq酶,,dNTPs和校正染料ROX,。進行實驗時,PCR反應(yīng)液的配制十分方便簡單,,只須將2×SG Green qPCR Mix稀釋至終濃度1×,,在反應(yīng)體系中加入模板和引物即可,。具有快速、靈敏度高,、特異性強,、穩(wěn)定性好等優(yōu)點。本制品適合于快速real-time PCR的擴增反應(yīng),,可以在寬廣的定量區(qū)域內(nèi)得到良好的標準曲線,,對目的基因進行準確定量,重復(fù)性好,。熒光染料SYBR® Green I僅與雙鏈DNA結(jié)合后發(fā)出熒光,,通過檢測反應(yīng)體系中的SYBR® Green I熒光強度,達到實時檢測PCR產(chǎn)物擴增量的目的,,可以對目的基因進行準確定量,。
產(chǎn)品儲存條件
1. -20℃,一年有效,;經(jīng)常使用,,可放于4℃。
發(fā)表文獻精選:
[1] IF=2.323 Ovarian Circular RNAs Associated with High and Low
Fertility in Large White Sows during the Follicular
and Luteal Phases of the Estrous Cycle;Animals 2020, 10(4), 696
[2] IF=2.985 A zebrafish (danio rerio) model for high-throughput screening food
and drugs with uric acid-lowering activity; Biochemical and Biophysical Research Communications 508 (2019) 494e498
[3] IF=3.191 Genome-Wide DNA Methylation Profile
of Gene cis-Acting Element Methylations
in All-trans Retinoic Acid-Induced Mouse Cleft Palate; DNA AND CELL BIOLOGY
Volume 37, Number 12, 2018
[4] IF=2.274 Analysis of long non-coding RNA expression profiles following focal cerebral
ischemia in mice; Neuroscience Letters 665 (2018) 123–129
[5] IF=2.773 Accumulating mechanism of γ-aminobutyric acid in soybean
(Glycine max L.) during germination; International Journal of Food Science and Technology 2018, 53, 106–111
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