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锟絘品锟斤拷锟斤拷
锟斤拷锟斤拷司RIPA Buffer锟絤锟斤拷锟节從革拷锟絅锟剿癸拷锟斤拷锟紹(y锟斤拷ng)锟侥硷拷锟斤拷锟酵革拷锟絅锟斤拷锟斤拷锟斤拷飳嶏拷w锟組锟斤拷锟斤拷锟斤拷锟絏锟斤拷锟斤拷锟斤拷,锟斤拷锟今經結,锟斤拷锟斤拷锟終锟斤拷锟斤拷锟斤拷锟斤拷锟饺ｏ拷锟斤拷锟斤拷取锟斤拷锟斤拷锟斤拷,锟斤拷锟斤拷取锟絕锟教猴拷锟轿凤拷锟斤拷,锟斤拷锟斤拷锟斤拷1小锟絩锟斤拷锟斤拷锟,锟斤拷該試锟斤拷锟斤拷取锟侥匡拷锟斤拷锟阶匡拷锟斤拷锟斤拷Western Blot锟斤拷锟斤拷锟斤拷锟絴锟接,锟斤拷锟斤拷锟竭癸拷锟斤拷锟斤拷锟斤拷锟斤拷锟轿碉拷锟斤拷锟叫撅拷,锟斤拷锟斤拷锟结蛋锟阶匡拷锟斤拷BCA锟斤拷Bradford锟饺凤拷锟斤拷锟統锟斤拷锟斤拷锟阶濓拷锟,锟斤拷
锟絘品锟斤拷要锟組锟街和冿拷锟斤拷l锟斤拷
1. 锟絘品锟斤拷要锟組锟街ｏ拷Tris,锟斤拷NaCl锟斤拷NP-40,锟斤拷SDS,锟斤拷
2. 4锟芥保锟芥，一锟斤拷锟斤拷效,锟斤拷
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